Advertisement

Applied Microbiology and Biotechnology

, Volume 46, Issue 3, pp 313–317 | Cite as

Degradation of anthracene by laccase of Trametes versicolor in the presence of different mediator compounds

  • C. Johannes
  • A. Majcherczyk
  • A. Hüttermann
ORIGINAL PAPER

Abstract

 Laccase of Trametes versicolor was generally able to oxidize anthracene in vitro. After 72 h incubation about 35% of the anthracene was transformed stoichiometrically to 9,10-anthraquinone. Transformation of anthracene increased rapidly in the presence of different mediators that readily generate stable radicals: 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 1-hydroxybenzotriazole. For the reaction, the presence of both the laccase and the mediator was necessary. In the presence of 0.005 mM 1-hydroxybenzotriazole this conversion had removed 47% of the anthracene after 72 h; 75% of the substrate was oxidized during this period when ABTS (1 mM) was used as mediator. In contrast to reactions without or with only low concentrations of a mediator, there was a discrepancy between the disappearance of anthracene and the formation of 9,10-anthraquinone in mediator-forced reactions. Coupling-products of mediators with anthracene degradation products were found. Anthracene disappeared nearly completely after incubation for 72 h with laccase in a 0.1 mM solution of 1-hydroxybenzotriazole and was transformed to 9,10-anthraquinone in about 80% yield; 90% of the substrate was transformed in the presence of ABTS (2.0 mM) resulting again in 80% quinone. Phenothiazine was not effective in this system.

Keywords

Quinone Degradation Product Anthracene Phenothiazine Stable Radical 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • C. Johannes
    • 1
  • A. Majcherczyk
    • 1
  • A. Hüttermann
    • 1
  1. 1.Institute of Forest Botany, University of Göttingen, Büsgenweg 2, 37077 Göttingen, Germany. Fax: +49 551 39 27 05 E-mail: amajche@gwdg.deDE

Personalised recommendations