Applied Microbiology and Biotechnology

, Volume 46, Issue 3, pp 313–317 | Cite as

Degradation of anthracene by laccase of Trametes versicolor in the presence of different mediator compounds

  • C. Johannes
  • A. Majcherczyk
  • A. Hüttermann
ORIGINAL PAPER

Abstract

 Laccase of Trametes versicolor was generally able to oxidize anthracene in vitro. After 72 h incubation about 35% of the anthracene was transformed stoichiometrically to 9,10-anthraquinone. Transformation of anthracene increased rapidly in the presence of different mediators that readily generate stable radicals: 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 1-hydroxybenzotriazole. For the reaction, the presence of both the laccase and the mediator was necessary. In the presence of 0.005 mM 1-hydroxybenzotriazole this conversion had removed 47% of the anthracene after 72 h; 75% of the substrate was oxidized during this period when ABTS (1 mM) was used as mediator. In contrast to reactions without or with only low concentrations of a mediator, there was a discrepancy between the disappearance of anthracene and the formation of 9,10-anthraquinone in mediator-forced reactions. Coupling-products of mediators with anthracene degradation products were found. Anthracene disappeared nearly completely after incubation for 72 h with laccase in a 0.1 mM solution of 1-hydroxybenzotriazole and was transformed to 9,10-anthraquinone in about 80% yield; 90% of the substrate was transformed in the presence of ABTS (2.0 mM) resulting again in 80% quinone. Phenothiazine was not effective in this system.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • C. Johannes
    • 1
  • A. Majcherczyk
    • 1
  • A. Hüttermann
    • 1
  1. 1.Institute of Forest Botany, University of Göttingen, Büsgenweg 2, 37077 Göttingen, Germany. Fax: +49 551 39 27 05 E-mail: amajche@gwdg.deDE

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