Applied Microbiology and Biotechnology

, Volume 46, Issue 3, pp 220–225 | Cite as

Depolymerization of low-rank coal by extracellular fungal enzyme systems.

I. Screening for low-rank-coal-depolymerizing activities
  • M. Hofrichter
  • W. Fritsche
ORIGINAL PAPER

Abstract

 A miniscale screening system was developed to detect depolymerizing activities of fungi toward low-rank coals. This system was suitable for the determination of changes in molecular masses as well as for the measurement of the enzymes responsible. A total of 486 fungal strains of different ecophysiological and taxonomic groups were tested for their ability to decolorize agar media containing coal-derived humic acids; 38 wood- and litter-decaying basidiomycetes caused a strong bleaching effect and 49 a weak effect. In contrast, micromycetes were proved to be unable to decolorize the coal substances. The wood-decaying fungus Nematoloma frowardii b19 most effectively bleached the medium. It could be demonstrated by gel-permeation chromatography that the strain really depolymerizes the high molecular-mass fractions of coal humic acids by forming fulvic-acid-like compounds. Extracellular enzyme activities of oxidases and peroxidases towards 2,2′-azinobis(3-ethylbenzthiazolinesulphonate) were extractable from the agar media.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • M. Hofrichter
    • 1
  • W. Fritsche
    • 1
  1. 1.Friedrich-Schiller-University Jena Institute for Microbiology, Department Technical Microbiology, Philosophenweg 12, D-07743 Jena, GermanyDE

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