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Applied Microbiology and Biotechnology

, Volume 46, Issue 2, pp 187–190 | Cite as

Fermentation of molasses using a thermotolerant yeast, Kluyveromyces marxianus IMB3: simplex optimisation of media supplements

  • S. Gough
  • O. Flynn
  • C. J. Hack
  • R. Marchant
SHORT CONTRIBUTION

Abstract

 The use of molasses as a substrate for ethanol production by the thermotolerant yeast Kluyveromyces marxianus var. marxianus was investigated at 45°C. A maximum ethanol concentration of 7.4% (v/v) was produced from unsupplemented molasses at a concentration of 23% (v/v). The effect on ethanol production of increasing the sucrose concentration in 23% (v/v) molasses was determined. Increased sucrose concentration had a similar detrimental effect on the final ethanol produced as the increase in molasses concentration. This indicated that the effect may be due to increased osmotic activity as opposed to other components in the molasses. The optimum concentration of the supplements nitrogen, magnesium, potassium and fatty acid for maximum ethanol production rate was determined using the Nelder and Mead (Computer J 7:308–313, 1965) simplex optimisation method. The optimum concentrations of the supplements were 0.576 g l-1 magnesium sulphate, 0.288 g l-1 potassium dihydrogen phosphate and 0.36% (v/v) linseed oil. Added nitrogen in the form of ammonium sulphate did not affect the ethanol production rate.

Keywords

Ethanol Production Ammonium Sulphate Sucrose Concentration Dihydrogen Phosphate Molasses 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • S. Gough
    • 1
  • O. Flynn
    • 1
  • C. J. Hack
    • 1
  • R. Marchant
    • 1
  1. 1.Biotechnology Research Group, School of Applied Biological and Chemical Sciences, University of Ulster, Coleraine, Co. Londonderry, BT52 1SA, Northern Ireland. Fax: 01265 324906, e-mail: S.Gough@ulst.ac.ukIE

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