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Applied Microbiology and Biotechnology

, Volume 46, Issue 2, pp 163–168 | Cite as

Cometabolic degradation of 4-chlorophenol by Alcaligenes eutrophus

  • G. A. Hill
  • B. J. Milne
  • P. A. Nawrocki
ORIGINAL PAPER

Abstract

Alcaligenes eutrophus was grown in batch cultures using either phenol as a sole substrate or mixtures of phenol and 4-chlorophenol. Phenol was found to be the sole source for carbon and energy while 4-chlorophenol was utilized only as a cometabolite. Maximum growth rates on phenol reached only 0.26 h-1, significantly below the growth rates reported earlier with Pseudomonas putida. The cometabolite was found to decrease biomass yield and increase lag time before logarithmic growth occurred. Both phenol and 4-chlorophenol were found to inhibit the growth rate linearly with maximum concentrations of 1080 ppm and 69 ppm respectively, beyond which no growth occurred. The best-fit parameters are incorporated into a simple, dynamic (i.e. time-varying) model capable of predicting all the batch growth conditions presented here. It is shown that P. putida is capable of faster bioremediation when phenol is the sole carbon source or for mixed substrates with low concentrations of the cometabolite, but for high concentrations of 4-chlorophenol, A. eutrophus becomes superior because of the long lag times that occur in the Pseudomonas species.

Keywords

Biomass Growth Rate Phenol Carbon Source Pseudomonas 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • G. A. Hill
    • 1
  • B. J. Milne
    • 1
  • P. A. Nawrocki
    • 1
  1. 1.Department of Chemical Engineering, 110 Science Place, University of Saskatchewan, Saskatoon, S7N 5C9, Canada. Fax: (306) 966 4777CA

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