Advertisement

Applied Microbiology and Biotechnology

, Volume 46, Issue 2, pp 106–111 | Cite as

Banana waste as substrate for α-amylase production by Bacillus subtilis (CBTK 106) under solid-state fermentation

  • C. Krishna
  • M. Chandrasekaran
ORIGINAL PAPER

Abstract

Bacillus subtilis CBTK 106, isolated from banana wastes, produced high titres of α-amylase when banana fruit stalk was used as substrate in a solid-state fermentation system. The effects of initial moisture content, particle size, cooking time and temperature, pH, incubation temperature, additional nutrients, inoculum size and incubation period on the production of α-amylase were characterised. A maximum yield of 5 345 000 U mg-1 min-1 was recorded when pretreated banana fruit stalk (autoclaved at 121 °C for 60 min) was used as substrate with 70% initial moisture content, 400 μm particle size, an initial pH of 7.0, a temperature of 35 °C, and additional nutrients (ammonium sulphate/sodium nitrate at 1.0%, beef extract/peptone at 0.5%, glucose/sucrose/starch/maltose at 0.1% and potassium chloride/sodium chloride at 1.0%) in the medium, with an inoculum-to-substrate ratio of 10% (v/w) for 24 h. The enzyme yield was 2.65-fold higher with banana fruit stalk medium compared to wheat bran.

Keywords

Maltose Bacillus Subtilis Bran Additional Nutrient Wheat Bran 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • C. Krishna
    • 1
  • M. Chandrasekaran
    • 1
  1. 1.Microbial Technology Unit, Centre for Biotechnology, Cochin University of Science and Technology, Cochin-682 022, Kerala, India. Fax: 91 484 532495IN

Personalised recommendations