Applied Microbiology and Biotechnology

, Volume 45, Issue 1–2, pp 162–168 | Cite as

Identification and production of a rhamnolipidic biosurfactant by a Pseudomonas species

  • S. Arino
  • R. Marchal
  • J. -P. Vandecasteele
Original Paper Applied Microbial and Cell Physiology


A glycolipid-producing bacterium, Pseudomonas aeruginosa GL1, was isolated from the soil contaminated with polycyclic aromatic hydrocarbons (PAH) from a manufactured gas plant. The glycolipid produced was characterized in detail by chromatographic procedures as a mixture of four rhamnolipids, consisting of different associations of rhamnose and hydroxy fatty acids: the main component was monorhamnosyl di-3-hydroxydecanoic acid. The rhamnolipid composition presented marked analogies with a defined part of P. aeruginosa outer membrane lipopolysaccharides (lipopolysaccharide band A). Rhamnolipid production was stimulated under conditions of nitrogen limitation. Glycerol yielded higher productions than did hydrophobic carbon sources. Cell hydrophobicity decreased during growth on glycerol and on n-hexadecane whereas glycolipid production increased. P. aeruginosa GL1 was found to be unable to grow on a variety of 2, 3 and 4 cycle PAH. However, it was shown to persist after at least 12 subcultures in a bacterial population growing on a mixture of pure PAH, suggesting a physiological role for rhamnolipid as a means to enhance PAH availability in a mutualistic PAH-degrading bacterial community.


Polycyclic Aromatic Hydrocarbon High Performance Liquid Chromatography Hydroxy Fatty Acid Rhamnolipids Rhamnolipid Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • S. Arino
    • 1
  • R. Marchal
    • 1
  • J. -P. Vandecasteele
    • 1
  1. 1.Division Chimie Appliquée, Biotechnologies, MatériauxInstitut Français du PétroleRueil-Malmaison CedexFrance

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