Applied Microbiology and Biotechnology

, Volume 45, Issue 1–2, pp 36–40 | Cite as

Lantibiotic nisin Z fermentative production by Lactococcus lactis IO-1: relationship between production of the lantibiotic and lactate and cell growth

  • H. Matsusaki
  • N. Endo
  • K. Sonomoto
  • A. Ishizaki
Original Paper Biotechnology


The influence of several parameters on the fermentative production of nisin Z by Lactococcus lactis IO-1 was studied. Considerable attention has been focused on the relationship between the primary metabolite production of bacteriocin and lactate and cell growth, which has so far not been clarified in detail. Production of nisin Z was optimal at 30°C and in the pH range 5.0–5.5. The addition of Ca2+ to the medium showed a stimulating effect on the production of nisin Z. A maximum activity of 3150 IU/ml was obtained during pH-controlled batch fermentation in the medium supplemented with 0.1 M CaCl2. It was about three times higher than that obtained under the optimal conditions for cell growth and lactic acid production.


Xylose Lactis Lactic Acid Bacterium Lactic Acid Production Lactococcus Lactis 
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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • H. Matsusaki
    • 1
  • N. Endo
    • 1
  • K. Sonomoto
    • 1
  • A. Ishizaki
    • 1
  1. 1.Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of AgricultureKyushu UniversityHigashi-ku, FukuokaJapan

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