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Applied Microbiology and Biotechnology

, Volume 53, Issue 2, pp 167–172 | Cite as

Production of polyhydroxyalkanoic acids by Ralstonia eutropha and Pseudomonas oleovorans from an oil remaining from biotechnological rhamnose production

  • B. Füchtenbusch
  • D. Wullbrandt
  • A. Steinbüchel
ORIGINAL ARTICLE

Abstract

Screening experiments identified several bacteria which were able to use residual oil from biotechnological rhamnose production as a carbon source for growth. Ralstonia eutropha H16 and Pseudomonas oleovorans were able to use this waste material as the sole carbon source for growth and for the accumulation of polyhydroxyalkanoic acids (PHA). R. eutropha and P. oleovorans accumulated PHA amounting to 41.3% and 38.9%, respectively, of the cell dry mass, when these strains were cultivated in mineral salt medium with the oil from the rhamnose production as the sole carbon source. The accumulated PHA isolated from R. eutropha consisted of only 3-hydroxybutyric acid, whereas the PHA isolated from P. oleovorans consisted of 3-hydroxyhexanoic acid, 3-hydroxyoctanoic acid, 3-hydroxy decanoic acid, and 3-hydroxydodecanoic acid. The composition was confirmed by gas chromatography of the isolated polyesters. Batch and fed-batch cultivations in stirred-tank reactors were done.

Keywords

Sole Carbon Source Waste Material Mineral Salt Rhamnose Salt Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • B. Füchtenbusch
    • 1
  • D. Wullbrandt
    • 2
  • A. Steinbüchel
    • 1
  1. 1.Institut für Mikrobiologie der Westfälischen Wilhelms-Universität Münster, Corrensstrasse 3, D-48149 Münster, Germany e-mail: steinbu@uni-muenster.de Tel.: +49-251-8339821 Fax: +49-251-8338388DE
  2. 2.Institut für Technologie der Kohlenhydrate – Zuckerinstitut – e.V., Langer Kamp 5, D-38106 Braunschweig, GermanyDE

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