Applied Microbiology and Biotechnology

, Volume 55, Issue 4, pp 423–427 | Cite as

Production of β-carotene by a mutant of Rhodotorula glutinis

  •  P. Bhosale
  •  R. Gadre
Original Paper

Abstract.

Wild strains of Rhodotorula glutinis and R. rubra were investigated concerning their carotenoid production, proportion of β-carotene and cell mass yield. R. glutinis NCIM 3353 produced 2.2 mg carotenoid/l in 72 h; and the amount of β-carotene was 14% (w/w) of the total carotenoid content (17 µg/g cell dry weight). It was subjected to mutagenesis using UV radiation for strain improvement. Out of 2,051 isolates screened, the yellow coloured mutant 32 produced 120-fold more β-carotene (2,048 µg/g cell dry weight) than the parent culture in 36 h, which was 82% (w/w) of the total carotenoid content. Mutant 32 was grown on different carbon and nitrogen sources. The best yield of β-carotene (33±3 mg/l) was obtained when glucose and yeast extract were supplied as carbon and nitrogen sources, respectively. Divalent cation salts further increased the total carotenoid content (66±2 mg/l) with β-carotene as the major component (55±2%, w/w).

Keywords

Carotenoid Nitrogen Source Cell Mass Good Yield Divalent Cation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  P. Bhosale
    • 1
  •  R. Gadre
    • 1
  1. 1.Chemical Engineering Division, National Chemical Laboratory, Pune 411 008, India

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