Applied Microbiology and Biotechnology

, Volume 55, Issue 3, pp 369–373

Biodegradation of tert-butyl alcohol and related xenobiotics by a methylotrophic bacterial isolate

  •  P. Piveteau
  •  F. Fayolle
  •  J.-P. Vandecasteele
  •  F. Monot
Short Contribution

Abstract.

A new aerobic bacterial strain, CIP I-2052, isolated from an activated sludge sample, was able to use tert-butyl alcohol (TBA), a product of methyl tert-butyl ether (MTBE) and ethyl tert-butyl ether (ETBE) degradation, as its sole carbon and energy source. Cobalt ions stimulated TBA mineralization. The maximum growth and TBA degradation rates were 0.032±0.004 h–1 and 35.8±8.5 mg TBA·g–1 (cell dry mass) per h, respectively. The growth yield on TBA was 0.54±0.02 g·g–1. Strain CIP I-2052 exhibited a particular substrate specificity towards alcohols. It degraded tertiary alcohols, TBA and tert-amyl alcohol (TAA), but neither their primary and secondary alcohol homologues, nor ethanol. However, one-carbon compounds, namely methanol and formate, were degraded by strain CIP I-2052, showing the methylotrophic nature of this isolate. The properties of this new strain suggest that it could be used for bioremediation of contaminated aquifers.

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  P. Piveteau
    • 1
  •  F. Fayolle
    • 1
  •  J.-P. Vandecasteele
    • 1
  •  F. Monot
    • 1
  1. 1.Institut Français du Pétrole, Département de Microbiologie, 1–4, avenue de Bois-Préau, 92852 Rueil-Malmaison Cedex, France

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