Biodegradation of tert-butyl alcohol and related xenobiotics by a methylotrophic bacterial isolate
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A new aerobic bacterial strain, CIP I-2052, isolated from an activated sludge sample, was able to use tert-butyl alcohol (TBA), a product of methyl tert-butyl ether (MTBE) and ethyl tert-butyl ether (ETBE) degradation, as its sole carbon and energy source. Cobalt ions stimulated TBA mineralization. The maximum growth and TBA degradation rates were 0.032±0.004 h–1 and 35.8±8.5 mg TBA·g–1 (cell dry mass) per h, respectively. The growth yield on TBA was 0.54±0.02 g·g–1. Strain CIP I-2052 exhibited a particular substrate specificity towards alcohols. It degraded tertiary alcohols, TBA and tert-amyl alcohol (TAA), but neither their primary and secondary alcohol homologues, nor ethanol. However, one-carbon compounds, namely methanol and formate, were degraded by strain CIP I-2052, showing the methylotrophic nature of this isolate. The properties of this new strain suggest that it could be used for bioremediation of contaminated aquifers.
KeywordsActivate Sludge Secondary Alcohol Tertiary Alcohol ETBE Activate Sludge Sample
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