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Applied Microbiology and Biotechnology

, Volume 54, Issue 6, pp 819–825 | Cite as

Mineralisation of 14C-labelled synthetic lignin and ligninolytic enzyme activities of litter-decomposing basidiomycetous fungi

  • K. T. Steffen
  • M. Hofrichter
  • A. Hatakka
ORIGINAL PAPER

Abstract

Within a screening program, 27 soil litter-decomposing basidiomycetes were tested for ligninolytic enzyme activities using agar-media containing 2,2′-azinobis(3-ethylbenzthiazoline-6-sulphonate), a humic acid or Mn2+ ions as indicator substrates. Most active species were found within the family Strophariaceae (Agrocybe praecox, Stropharia coronilla, S. rugosoannulata) and used for mineralisation experiments with a 14C-ring-labelled synthetic lignin (14C-DHP). The fungi mineralised around 25% of the lignin to 14CO2 within 12 weeks of incubation in a straw environment; about 20% of the lignin was converted to water-soluble fragments. Mn-peroxidase was found to be the predominant ligninolytic enzyme of all three fungi in liquid culture and its production was strongly enhanced in the presence of Mn2+ ions. The results of this study demonstrate that certain ubiquitous litter-decomposing basidiomycetes possess ligninolytic activities similar to the wood-decaying white-rot fungi, the most efficient lignin degraders in nature.

Keywords

Lignin Straw Humic Acid Screening Program Ligninolytic Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • K. T. Steffen
    • 1
  • M. Hofrichter
    • 1
  • A. Hatakka
    • 1
  1. 1.Department of Applied Chemistry and Microbiology, P.O. Box 56, 00014 University of Helsinki, Helsinki, Finland e-mail: Kari.Steffen@Helsinki.Fi Fax: +358-9-19159322FI

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