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Applied Microbiology and Biotechnology

, Volume 55, Issue 1, pp 61–68 | Cite as

Molecular cloning and characterization of a multidomain endoglucanase from Paenibacillus sp BP-23: evaluation of its performance in pulp refining

  • F. I. J. Pastor
  • X. Pujol
  • A. Blanco
  • T. Vidal
  • A. L. Torres
  • P. Díaz
ORIGINAL PAPER

Abstract

The gene celB encoding an endoglucanase from Paenibacillus sp. BP-23 was cloned and expressed in Escherichia coli. The nucleotide sequence of a 4161 bp DNA fragment containing the celB gene was determined, revealing an open reading frame of 2991 nucleotides that encodes a protein of 106,927 Da. Comparison of the deduced amino acid sequence of endoglucanase B with known β-glycanase sequences showed that the encoded enzyme is a modular protein and exhibits high homology to enzymes belonging to family 9 cellulases. The celB gene product synthesized in E. coli showed high activity on carboxymethyl cellulose and lichenan while low activity was found on Avicel. Activity was enhanced in the presence of 10 mM Ca2+ and showed its maximum at 53 °C and pH 5.5. The effect of the cloned enzyme in modifying the physical properties of pulp and paper from Eucalyptus was tested (CelB treatment). An increase in mechanical strength of paper and a decrease in pulp dewatering properties were found, indicating that CelB treatment can be considered as a biorefining. Treatment with CelB gave rise to an improvement in paper strength similar to that obtained with 1,000 revolutions increase in mechanical refining. Comparison with the performances of recently developed endoglucanase A from the same strain and with a commercial cellulase showed that CelB produced the highest refining effect.

Keywords

Enzyme Cellulose Cellulase Eucalyptus Deduce Amino Acid Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • F. I. J. Pastor
    • 1
  • X. Pujol
    • 1
  • A. Blanco
    • 1
  • T. Vidal
    • 2
  • A. L. Torres
    • 2
  • P. Díaz
    • 1
  1. 1.Department of Microbiology, Faculty of Biology, University of Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain e-mail: fpastor@bio.ub.es Tel.: +34-3-4034626 Fax: +34-3-4034629ES
  2. 2.Department of Textile and Paper Engineering, ETSII Terrassa, Polytechnic University of Catalonia, Colón 11, 08222 Terrassa, Barcelona, SpainES

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