Applied Microbiology and Biotechnology

, Volume 53, Issue 6, pp 661–667 | Cite as

A comparison of enzyme-aided bleaching of softwood paper pulp using combinations of xylanase, mannanase and α-galactosidase

  • J. H. Clarke
  • K. Davidson
  • J. E. Rixon
  • J. R. Halstead
  • M. P. Fransen
  • H. J. Gilbert
  • G. P. Hazlewood
ORIGINAL PAPER

Abstract

Enzymatic pretreatment of softwood kraft pulp was investigated using xylanase A (XylA) from Neocallimastix patriciarum in combination with mannanase and α-galactosidase. Mannanase A (ManA) from Pseudomonas fluorescens subsp. cellulosa and ManA from Clostridium thermocellum, both family 26 glycosyl hydrolases, are structurally diverse and exhibit different pH and temperature optima. Although neither mannanase was effective in pretreating softwood pulp alone, both enzymes were able to enhance the production of reducing sugar and the reduction of single-stage bleached κ number when used with the xylanase. Sequential incubations with XylA and P. fluorescens ManA produced the largest final κ number reduction in comparison to control pretreated pulp. The release of galactose from softwood pulp by α-galactosidase A (AgaA) from P. fluorescens was enhanced by the presence of ManA from the same microorganism, and a single pretreatment with these enzymes, in combination with XylA, gave the most effective κ number reduction using a single incubation. Results indicated that mixtures of hemicellulase activities can be chosen to enhance pulp bleachability.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • J. H. Clarke
    • 1
  • K. Davidson
    • 1
  • J. E. Rixon
    • 2
  • J. R. Halstead
    • 1
  • M. P. Fransen
    • 1
  • H. J. Gilbert
    • 2
  • G. P. Hazlewood
    • 3
  1. 1.Department of Cellular Physiology, The Babraham Institute, Babraham, Cambridge CB2 4AT, UKGB
  2. 2.Department of Biological and Nutritional Sciences, The Agriculture Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU, UKGB
  3. 3.Finnfeeds, P.O. Box 777, Marlborough, Wiltshire, SN8 1XN, UKGB

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