Abstract
Adaptive laboratory evolution is a powerful tool for microorganism improvement likely to produce enhanced microalgae better tailored to their industrial uses. In this work, 12 wild-type strains of Tisochrysis lutea were co-cultivated under increasing thermal stress for 6 months. Indeed, temperature was oscillating daily between a high and a low temperature, with increasing amplitude along the experiment. The goal was to enhance the polyunsaturated fatty acid content of the polar lipids. Samples were taken throughout the evolution experiment and cultivated in standardized conditions to analyze the evolution of the lipid profile. Genomic analysis of the final population shows that two strains survived. The lipid content doubled, impacting all lipid classes. The fatty acid analyses show a decrease in SFAs correlated with an increase in monounsaturated fatty acids (MUFAs), while changes in polyunsaturated fatty acid (PUFAs) vary between both photobioreactors. Hence, the proportion of C18-MUFAs (18:1 n-9) and most C18-PUFAs (18:2 n-6, 18:3 n-3, and 18:4 n-3) increased, suggesting their potential role in adjusting membrane fluidity to temperature shifts. Of particular interest, DHA in polar lipids tripled in the final population while the growth rate was not affected.
Key points
• Adaptive laboratory evolution on a mix of 12 T. lutea strains led to survival of 2
• Thermal stress impacted cell size, total lipid cell content, and all lipid classes
• DHA cell content partitioned to polar lipids tripled throughout the experiment
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Acknowledgments
This work was performed, in partnership with the SAS PIVERT, within the frame of the French Institute for the Energy Transition (Institut pour la Transition Energétique (ITE) P.I.V.E.R.T. (www.institut-pivert.com) selected as an Investment for the Future (“Investissements d’Avenir”). This work was supported, as part of the Investments for the Future, by the French Government under the reference ANR-001-01.
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This study was funded by P.I.V.E.R.T. (GENESYS program - WP4P5-73-01).
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MG, AT, and EP designed and conducted the experiments. MG, MB, and GC analyzed data. MG wrote the manuscript. GC, FG, OB, and AS corrected the manuscript. All authors read and approved the manuscript.
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Gachelin, M., Boutoute, M., Carrier, G. et al. Enhancing PUFA-rich polar lipids in Tisochrysis lutea using adaptive laboratory evolution (ALE) with oscillating thermal stress. Appl Microbiol Biotechnol 105, 301–312 (2021). https://doi.org/10.1007/s00253-020-11000-4
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DOI: https://doi.org/10.1007/s00253-020-11000-4