Combination of the CRP mutation and ptsG deletion in Escherichia coli to efficiently synthesize xylitol from corncob hydrolysates

  • Xinsong Yuan
  • Shuai Tu
  • Jianping Lin
  • Lirong Yang
  • Huahao Shen
  • Mianbin WuEmail author
Biotechnological products and process engineering


The biotechnology-based production of xylitol has received widespread attention because it can use cheap and renewable lignocellulose as a raw material, thereby decreasing costs and pollution. The simultaneous use of various sugars in lignocellulose hydrolysates is a primary prerequisite for efficient xylitol production. In this study, a ΔptsG and crp* combinatorial strategy was used to generate Escherichia coli W3110 strain IS5-dI, which completely eliminated glucose repression and simultaneously used glucose and xylose. This strain produced 164 g/L xylitol from detoxified corncob hydrolysates during a fed-batch fermentation in a 15-L bioreactor, which was 14.7% higher than the xylitol produced by the starting strain, IS5-d (143 g/L), and the xylitol productivity was 3.04 g/L/h. These results represent the highest xylitol concentration and productivity reported to date for bacteria and hemicellulosic sugars. Additionally, strain IS5-dG, which differs from IS5-dI at CRP amino acid residue 127 (I127G), was tolerant to the toxins in corncob hydrolysates. In a fed-batch fermentation experiment involving a 15-L bioreactor, IS5-dG produced 137 g/L xylitol from non-detoxified corncob hydrolysates, with a productivity of 1.76 g/L/h. On the basis of these results, we believe that IS5-dI and IS5-dG may be useful host strains for the industrial-scale production of xylitol from detoxified or non-detoxified corncob hydrolysates.


Xylitol CCR CRP CRISPR/Cas9 Non-detoxified Hemicellulosic hydrolysate 


Funding information

This study was funded by the National Natural Science Foundation of China (21376215) and the National Key R&D Program of China (2018YFC1604102).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics statement

The research described in this article did not involve human participants or animals.

Supplementary material

253_2019_10324_MOESM1_ESM.pdf (626 kb)
ESM 1 (PDF 625 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2020

Authors and Affiliations

  • Xinsong Yuan
    • 1
  • Shuai Tu
    • 2
  • Jianping Lin
    • 1
  • Lirong Yang
    • 1
  • Huahao Shen
    • 3
  • Mianbin Wu
    • 1
    Email author
  1. 1.Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological EngineeringZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.School of Chemistry and Chemical EngineeringHefei Normal UniversityHefeiPeople’s Republic of China
  3. 3.Department of Respiratory and Critical Care Medicine, Second Affiliated HospitalZhejiang University School of MedicineHangzhouPeople’s Republic of China

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