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Applied Microbiology and Biotechnology

, Volume 104, Issue 3, pp 1243–1257 | Cite as

Effects of a probiotic intervention on Escherichia coli and high-fat diet-induced intestinal microbiota imbalance

  • Qingshen Sun
  • Song Zhang
  • Xinyang Liu
  • Yuting Huo
  • Bin Su
  • Xiuliang LiEmail author
Applied microbial and cell physiology
  • 132 Downloads

Abstract

Obesity is often associated with intestinal microbiota imbalance and increased Gram-negative bacteria characterized by higher endotoxin levels. Therefore, a study on the joint effects of a high-fat diet and Gram-negative bacteria prechallenge might provide further information on the interactive effects of intestinal microbiota and obesity as well as the effects of a probiotic intervention on these processes. This study focused on the joint effects of a high-fat diet and Escherichia coli on mouse inflammatory cytokines, intestinal microbiota, hepatic pathological changes, and the alleviating capacity of probiotics (Lactobacillus plantarum, Bifidobacterium breve, and Lactobacillus fermentum). Our results showed that E. coli administration and the high-fat diet exacerbated the inflammatory syndrome by increasing the visceral fat content, the inflammatory cell infiltration, and intestinal microbiota disorder in mice. E. coli administration caused a decrease in short-chain fatty acids in mouse feces, and probiotics effectively improved this phenomenon. Compound probiotic intervention reduced LPS and IL-1β levels, while increased IL-10 levels in mice improved the degeneration and inflammatory infiltration of mouse liver cells. The intestinal microbiota showed great differences at 3 weeks and 6 weeks post-administration. High fat and E. coli alone or in combination caused intestinal microbiota disorder, with increased harmful bacteria, and the probiotics effectively improved the intestinal flora structure and increased the fecal short-chain fatty acid (SCFA) content. In conclusion, a high-fat diet and Gram-negative bacteria challenge exacerbated the inflammatory syndrome, which can be alleviated by compound probiotic intervention.

Keywords

High-fat diet Inflammation Escherichia coli Compound probiotics Intestinal microbiota 

Notes

Funding information

This study was funded by the Heilongjiang Province Natural Science Foundation of China (C2016049) and the Harbin City Technology Bureau Youth Talented Person Project (RC2017QN020010).

Compliance with ethical standards

Animal experiments were carried out in compliance with the guidelines for the care and use of laboratory animals and the institutional ethical guidelines in China.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

253_2019_10304_MOESM1_ESM.pdf (139 kb)
ESM 1 (PDF 138 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Engineering Research Center of Agricultural Microbiology Technology, Ministry of EducationHeilongjiang UniversityHarbinChina
  2. 2.Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life SciencesHeilongjiang UniversityHarbinChina
  3. 3.Key Laboratory of Molecular Biology, College of Heilongjiang Province, School of Life SciencesHeilongjiang UniversityHarbinChina

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