Sulfur-containing amino acid supplementation to gilts from late pregnancy to lactation altered offspring’s intestinal microbiota and plasma metabolites
- 61 Downloads
Maternal nutrition during late pregnancy and lactation is highly involved with the offspring’s health status. The study was carried out to evaluate the effects of different ratios of methionine and cysteine (Met/Cys: 46% Met, 51% Met, 56% Met, and 62% Met; maintained with 0.78% of total sulfur-containing amino acids; details in “Materials and methods”) supplements in the sows’ diet from late pregnancy to lactation on offspring’s plasma metabolomics and intestinal microbiota. The results revealed that the level of serum albumin, calcium, iron, and magnesium was increased in the 51% Met group compared with the 46% Met, 56% Met, and 62% Met groups. Plasma metabolomics results indicated that the higher ratios of methionine and cysteine (0.51% Met, 0.56% Met, and 0.62% Met)–supplemented groups enriched the level of hippuric acid, retinoic acid, riboflavin, and δ-tocopherol than in the 46% Met group. Furthermore, the 51% Met–supplemented group had a higher relative abundance of Firmicutes compared with the other three groups (P < 0.05), while the 62% Met–supplemented group increased the abundance of Proteobacteria compared with the other three groups (P < 0.05) in piglets’ intestine. These results indicated that a diet consisting with 51% Met is the optimum Met/Cys ratio from late pregnancy to lactation can maintain the offspring’s health by improving the serum biochemical indicators and altering the plasma metabolomics profile and intestinal gut microbiota composition, but higher proportion of Met/Cys may increase the possible risk to offspring’s health.
KeywordsSulfur-containing amino acids Pregnancy Lactation Intestinal microbiota Plasma metabolomics
The authors acknowledge the CAS-TWAS President’s Fellowship for international PhD students.
This study was supported by National Natural Science Foundation of China (No. 31772642, 31672457), International Partnership Program of Chinese Academy of Sciences (161343KYSB20160008), Hunan Provincial Science and Technology Department (2017NK2322, 2018CT5002, 2018WK4025), Local Science and Technology Development Project guided by The Central Government (YDZX20184300002303), Double first-class construction project of Hunan Agricultural University (SYL201802003, YB2018007, CX20190497), Project for Yunnan Yin Yulong Academician Workstation from Yunan Province (2018IC087), and China Postdoctoral Science Foundation (2018 M632963, 2019 T120705).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The study was carried out in accordance with the guidelines of the Laboratory Animal Ethical Commission of the Chinese Academy of Science and approved by the Animal Care Committee of the Institute of Subtropical Agriculture (201703-64C), Chinese Academy of Science, Changsha, China.
- Aiello SE (2016) Serum proteins and the dysproteinmias. The Merk veterinary manual. Merk & Co. Inc, Kenilworth, pp 3173–3174Google Scholar
- da Silva BP, Toledo RCL, Grancieri M, Moreira MEC, Medina NR, Silva RR, Costa NMB, Martino HSD (2019) Effects of chia (Salvia hispanica L.) on calcium bioavailability and inflammation in Wistar rats. Food Res Int 116:592–599. https://doi.org/10.1016/j.foodres.2018.08.078 CrossRefPubMedGoogle Scholar
- Dallanora D, Marcon J, Walter MP, Biondo N, Bernardi ML, Wentz I, Bortolozzo FP (2017) Effect of dietary amino acid supplementation during gestation on placental efficiency and litter birth weight in gestating gilts. Livest Sci 197:30–35. https://doi.org/10.1016/j.livsci.2017.01.005 CrossRefGoogle Scholar
- Fontana M, Pecci L, Duprè S, Cavallini DJNR (2004) Antioxidant properties of sulfinates: protective effect of hypotaurine on peroxynitrite-dependent damage. Neurochem Res 29(1):111–116. https://doi.org/10.1023/B:NERE.0000010439.99991.cf CrossRefPubMedGoogle Scholar
- Fukumori C, Casaro MB, Thomas AM, Mendes E, Ribeiro WR, Crisma AR, Murata GM, Bizzarro B, Dias-Neto E, Setubal JC, Oliveira MA, Tavares-de-Lima W, Curi R, Bordin S, Sartorelli P, Ferreira CM (2019) Maternal supplementation with a synbiotic has distinct outcomes on offspring gut microbiota formation in A/J and C57BL/6 mice, differentially affecting airway inflammatory cell infiltration and mucus production. J Funct Foods 61. https://doi.org/10.1016/j.jff.2019.103496 CrossRefGoogle Scholar
- Huang C, Chiba LI, Magee WE, Wang Y, Griffing DA, Torres IM, Rodning SP, Bratcher CL, Bergen WG, Spangler EA (2019) Effect of flaxseed oil, animal fat, and vitamin E supplementation on growth performance, serum metabolites, and carcass characteristics of finisher pigs, and physical characteristics of pork. Livest Sci 220:143–151. https://doi.org/10.1016/j.livsci.2018.11.011 CrossRefGoogle Scholar
- Janowski A, Kolb R, Zhang W, Sutterwala F (2013) Beneficial and detrimental roles of NLRs in carcinogenesis. Front Immunol 4(370). https://doi.org/10.3389/fimmu.2013.00370
- Liang H, Dai Z, Kou J, Sun K, Chen J, Yang Y, Wu G, Wu Z (2018) Dietary L-tryptophan supplementation enhances the intestinal mucosal barrier function in weaned piglets: implication of tryptophan-metabolizing microbiota. Int J Mol Sci 20(1). https://doi.org/10.3390/ijms20010020 CrossRefGoogle Scholar
- Liu D, Zong EY, Huang PF, Yang HS, Yan SL, Li JZ, Li YL, Ding XQ, He SP, Xiong X, Yin YL (2019) The effects of dietary sulfur amino acids on serum biochemical variables, mucosal amino acid profiles, and intestinal inflammation in weaning piglets. Livest Sci 220:32–36. https://doi.org/10.1016/j.livsci.2018.12.013 CrossRefGoogle Scholar
- MacKay DS, Brophy JD, McBreairty LE, McGowan RA, Bertolo RF (2012) Intrauterine growth restriction leads to changes in sulfur amino acid metabolism, but not global DNA methylation, in Yucatan miniature piglets. J Nutr Biochem 23(9):1121–1127. https://doi.org/10.1016/j.jnutbio.2011.06.005 CrossRefPubMedGoogle Scholar
- Martin Agnoux A, Antignac JP, Boquien CY, David A, Desnots E, Ferchaud-Roucher V, Darmaun D, Parnet P, Alexandre-Gouabau MC (2015) Perinatal protein restriction affects milk free amino acid and fatty acid profile in lactating rats: potential role on pup growth and metabolic status. J Nutr Biochem 26(7):784–795. https://doi.org/10.1016/j.jnutbio.2015.02.012 CrossRefPubMedGoogle Scholar
- Mennitti LV, Oliveira JL, Morais CA, Estadella D, Oyama LM, Oller do Nascimento CM, Pisani LP (2015) Type of fatty acids in maternal diets during pregnancy and/or lactation and metabolic consequences of the offspring. J Nutr Biochem 26(2):99–111. https://doi.org/10.1016/j.jnutbio.2014.10.001 CrossRefPubMedGoogle Scholar
- NRC (2012) Nutrient requirements of swine: eleventh revised edition. The National Academies Press, Washington, DC. https://doi.org/10.17226/13298
- Remus A, Hauschild L, Corrent E, Letourneau-Montminy MP, Pomar C (2019) Pigs receiving daily tailored diets using precision-feeding techniques have different threonine requirements than pigs fed in conventional phase-feeding systems. J Anim Sci Biotechnol 10:16. https://doi.org/10.1186/s40104-019-0328-7 CrossRefPubMedPubMedCentralGoogle Scholar
- Sabui S, Kapadia R, Ghosal A, Schneider M, Lambrecht NWG, Said HM (2018) Biotin and pantothenic acid oversupplementation to conditional SLC5A6 KO mice prevents the development of intestinal mucosal abnormalities and growth defects. Am J Physiol Cell Physiol 315(1):C73–C79. https://doi.org/10.1152/ajpcell.00319.2017 CrossRefPubMedPubMedCentralGoogle Scholar
- Traber MG (2012) Vitamin E, vol 11th Ed. Lippincott Williams & Wilkins, BaltimoreGoogle Scholar
- Trushina E, Dutta T, Persson XM, Mielke MM, Petersen RC (2013) Identification of altered metabolic pathways in plasma and CSF in mild cognitive impairment and Alzheimer's disease using metabolomics. PLoS One 8(5):e63644. https://doi.org/10.1371/journal.pone.0063644 CrossRefPubMedPubMedCentralGoogle Scholar
- Wei H, Zhao X, Xia M, Tan C, Gao J, Htoo JK, Xu C, Peng J (2019) Different dietary methionine to lysine ratios in the lactation diet: effects on the performance of sows and their offspring and methionine metabolism in lactating sows. J Anim Sci Biotechnol 10:76–11. https://doi.org/10.1186/s40104-019-0373-2 CrossRefPubMedPubMedCentralGoogle Scholar
- Zhong H, Li H, Liu G, Wan H, Mercier Y, Zhang X, Lin Y, Che L, Xu S, Tang L, Tian G, Chen D, Wu FZ (2016) Increased maternal consumption of methionine as its hydroxyl analog promoted neonatal intestinal growth without compromising maternal energy homeostasis. J Anim Sci Biotechnol 7:46. https://doi.org/10.1186/s40104-016-0103-y CrossRefPubMedPubMedCentralGoogle Scholar