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Applied Microbiology and Biotechnology

, Volume 104, Issue 3, pp 1291–1305 | Cite as

Characterization of an 17β-estradiol-degrading bacterium Stenotrophomonas maltophilia SJTL3 tolerant to adverse environmental factors

  • Weiliang Xiong
  • Chong Yin
  • Wanli Peng
  • Zixin Deng
  • Shuangjun Lin
  • Rubing LiangEmail author
Environmental biotechnology
  • 208 Downloads

Abstract

Bioremediation of environmental estrogens requires microorganisms with stable degradation efficiency and great stress tolerance in complex environments. In this work, Stenotrophomonas maltophilia SJTL3 isolated from wastewater was found to be able to degrade over 90% of 10 μg/mL 17β-estradiol (E2) in a week and the degradation dynamic was fitted by the first-order kinetic equations. Estrone was the first and major intermediate of E2 biodegradation. Strain SJTL3 exhibited strong tolerance to several adverse conditions like extreme pH (3.0–11.0), high osmolality (2%), co-existing heavy metals (6.25 μg/mL of Cu2+) and surfactants (5 CMC of Tween 80), and retained normal cell vitality and stable E2-degradaing efficiency. In solid soil, strain SJTL3 could remove nearly 100% of 1 μg/mL of E2 after the bacteria inoculation and 8-day culture. As to the contamination of 10 μg/mL E2 in soil, the biodegradation efficiency was about 90%. The further obtainment of the whole genome of strain SJTL3 and genome analysis revealed that this strain contained not only the potential genes responsible for estrogen degradation, but also the genes encoding proteins involved in stress tolerance. This work could promote the estrogen-biodegrading mechanism study and provide insights into the bioremediation application.

Keywords

Stenotrophomonas maltophilia SJTL3 Biodegradation 17β-estradiol Estrone Stress tolerance 

Notes

Acknowledgments

We acknowledge Shanghai Personal Biotech Co., Ltd. for genome sequencing.

Author contributions

R L designed the experiments; X L and R L wrote the manuscript. X L, C Y, and W P performed the experiments. D Z and L S gave support on the experiments. All the authors discussed the results and commented on the manuscript.

Funding information

This work was supported by the National Science Foundation of China (31570099) and the Natural Science Foundation of Shanghai (19ZR1475500).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

253_2019_10281_MOESM1_ESM.pdf (1000 kb)
ESM 1 (PDF 1000 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina

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