Comparative transcriptomics and transcriptional regulation analysis of enhanced laccase production induced by co-culture of Pleurotus eryngii var. ferulae with Rhodotorula mucilaginosa

  • Qi Zhang
  • Liting Zhao
  • YouRan Li
  • Feng Wang
  • Song Li
  • Guiyang Shi
  • Zhongyang DingEmail author
Genomics, transcriptomics, proteomics


The co-culturing of Pleurotus eryngii var. ferulae and Rhodotorula mucilaginosa was confirmed in our previous studies to be an efficient strategy to improve laccase production by submerged fermentation. To determine the possible regulation principles underlying this behaviour, comparative transcriptomic analysis was performed on P. eryngii var. ferulae to investigate the differential expression of genes in co-culture. RNA-seq analysis showed that genes concerning xenobiotic biodegradation and expenditure of energy were upregulated. However, genes related to oxidative stress were downregulated. In addition, the transcription levels of laccase isoenzymes were not consistent in the co-culture system: 3 laccase genes (lacc1, lacc2, lacc12) were upregulated, and 3 laccase genes (lacc4, lacc6, lacc9) were downregulated. The enhancement in laccase activity can be due to upregulation of a laccase heterodimer encoded by the genes lacc2 and ssPOXA3a (or ssPOXA3b), whose expression levels were increased by 459% and 769% (or 585% for ssPOXA3b) compared with those of a control, respectively. β-Carotene produced by R. mucilaginosa upregulated the transcription of lacc2 only. Combining these results with an analysis of cis-acting responsive elements indicated that four transcription factors (TFs) had potential regulatory effects on the transcription of laccase genes. It was supposed that TFa regulated lacc transcription by binding with methyl jasmonate and heat shock response elements. The expression of TFb, TFc, and TFd was regulated by β-carotene. However, β-carotene had no effect on TFa expression. These results provide a possible mechanism for the regulation of laccase gene transcription in the co-culture system and are also beneficial for the future intensification of fungal laccase production.


Laccase Co-culture β-Carotene RNA-seq Transcriptional regulation 



Funding for this study was provided by the National Natural Science Foundation of China (31571822) and the Science and Technology Project of Jiangsu Province (social development category, BE2017683). This study was also sponsored by the National First-Class Discipline Programme of Light Industry Technology and Engineering (LITE2018-22).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

This research did not involve any human participants.

Supplementary material

253_2019_10228_MOESM1_ESM.pdf (765 kb)
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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Qi Zhang
    • 1
    • 2
  • Liting Zhao
    • 2
  • YouRan Li
    • 1
    • 2
  • Feng Wang
    • 3
  • Song Li
    • 4
  • Guiyang Shi
    • 1
    • 2
  • Zhongyang Ding
    • 1
    • 2
    Email author
  1. 1.Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina
  2. 2.National Engineering Laboratory for Cereal Fermentation TechnologyJiangnan UniversityWuxiChina
  3. 3.School of Food and Biological EngineeringJiangsu UniversityZhenjiangChina
  4. 4.School of Biological and Chemical EngineeringAnhui Polytechnic UniversityWuhuChina

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