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Efficient stabilisation of curcumin microencapsulated into yeast cells via osmoporation

  • Fábio Gonçalves Macêdo de Medeiros
  • Sebastien Dupont
  • Laurent Beney
  • Gaëlle Roudaut
  • Roberta Targino Hoskin
  • Márcia Regina da Silva PedriniEmail author
Applied microbial and cell physiology

Abstract

This study proposes the investigation of curcumin encapsulation into Saccharomyces cerevisiae cells through osmoporation as an efficient way of increasing curcumin stability. The influence of three process parameters (cell, ethanol and curcumin concentrations) on the encapsulation process was evaluated, and the obtained biocapsules were characterised for physical and photochemical stabilisation. Results showed that encapsulation efficiency was favoured by the increase of cells/curcumin ratio and ethanol concentration up to 60%. Differential scanning calorimetry (DSC) curves revealed that yeast encapsulation delayed the curcumin melting point up to 207 °C. Encapsulated curcumin retained over 80% of antioxidant activity after thermal treatment (150 °C) and over 70% after a 50-day exposure to artificial light. Photochemical stability of yeast-encapsulated curcumin was increased by 5.7-fold, and half-life time reached 181 days under illumination conditions. Overall, osmoporation-produced yeast biocapsules confirmed the versatility of osmoporation as an encapsulation technique and successfully improved curcumin stability.

Keywords

Curcumin Osmoporation S. cerevisiae Antioxidant activity Stability Microencapsulation 

Notes

Acknowledgements

The authors would like to thank the Federal University of Rio Grande do Norte (UFRN), the Núcleo de Pesquisa em Alimentos e Medicamentos (NUPLAM/UFRN) and the Department of Material Science and Engineering (DEMAT/UFRN) for the technical support.

Funding information

F. G. M. de Medeiros was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), grant number 144415/2017-8.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Bioprocess Laboratory, Chemical Engineering DepartmentFederal University of Rio Grande do NorteNatalBrazil
  2. 2.Laboratory of Food Bioactive Compounds, Chemical Engineering DepartmentFederal University of Rio Grande do NorteNatalBrazil
  3. 3.UMR Procédés Alimentaires et Microbiologiques (PAM UMR A 02.102)Univ. Bourgogne Franche-Comté, AgroSup DijonDijonFrance

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