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Global identification of alternative splicing in Shiraia bambusicola and analysis of its regulation in hypocrellin biosynthesis

  • Xin-Yao Liu
  • Li Fan
  • Jian Gao
  • Xiao-Ye ShenEmail author
  • Cheng-Lin HouEmail author
Applied genetics and molecular biotechnology

Abstract

Hypocrellins, as natural pigments from Shiraia bambusicola, have extensive applications in the agricultural, cosmetic, food, and feed industries, and play a vital role in photodynamic therapy for anticancer and antiviral treatments. However, environmental stresses are always the bottlenecks for increasing hypocrellin yield during the process of fermentation. Pre-mRNA alternative splicing (AS) is an essential mechanism in the defense of abiotic stresses in the animal and plant kingdom, but is seldom involved in fungi. In this study, AS from genome-wide sequencing and RNA-seq data for S. bambusicola was analyzed for the first time. Interestingly, the proportion of AS in S. bambusicola was 38.44% (most of them participated in metabolic processes, covering pigmentation and response to stimulus), a much higher ratio than seen in that of other fungal species (1.3–18%). Here, we identified the relationship of AS and secondary metabolic (SM) biosynthesis under a series of abiotic stresses. Suitable fungicides suppressed hypocrellin production significantly, and AS occurred in key functional genes (sbFLO, sbMFS, sbPKS) of hypocrellin biosynthesis. In contrast, H2O2 improved the yield of hypocrellins, but AS were not found in the corresponding gene cluster. A further study showed that overexpressing an isoform of sbPKS (sbPKSa) in Shiraia bambusicola could dramatically down-regulate the expression of the original gene sbPKS and nearly inhibit the production of hypocrellins. Altogether, our study strongly supported the hypothesis that AS had a vital role in the regulation of hypocrellin biosynthesis under stresses, and initially explored whether SM functional genes were relevant for fungi.

Keywords

Alternative splicing (AS) Hypocrellin biosynthesis Fermentation Abiotic stresses Shiraia bambusicola 

Notes

Acknowledgment

We thank Dr. Gang Liu for kind supply of plasmid pAg1 and A. tumefaciens strain AGL-1, and also for the constructive advice.

Disclaimer

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Funding

This study was supported by the National Natural Science Foundation of China (nos. 31470145 and 31500015).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2019_10189_MOESM1_ESM.pdf (256 kb)
ESM 1 (PDF 256 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Life ScienceCapital Normal UniversityBeijingPeople’s Republic of China
  2. 2.Key Laboratory of Bamboo and Rattan Science and Technology of the SFAInternational Centre for Bamboo and RattanBeijingPeople’s Republic of China

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