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Applied Microbiology and Biotechnology

, Volume 103, Issue 17, pp 7129–7140 | Cite as

Biodegradation of mycotoxin fumonisin B1 by a novel bacterial consortium SAAS79

  • Zhiyong Zhao
  • Yanmei Zhang
  • Andong Gong
  • Na Liu
  • Shanshan Chen
  • Xiaoyan Zhao
  • Xiaobei Li
  • Lei Chen
  • Changyan Zhou
  • Jianhua WangEmail author
Applied microbial and cell physiology

Abstract

Fumonisin B1 (FB1) contamination in cereals and cereal products remains an important aspect of food safety because of its wide distribution and the potential health hazard. However, only a few microorganisms have been reported to effectively degrade FB1. In this present study, a bacterial consortium SAAS79 with highly FB1-degrading activity was isolated from the spent mushroom compost. The combination of antibiotic-driven selection and 16S rDNA sequencing identified the Pseudomonas genus as the key FB1-degrading member. The microbial consortium could degrade more than 90% of 10 μg/mL FB1 after incubation for 24 h at pH of 5–7 and temperature of 28–35 °C. The enzymes from the intracellular space were proved to be responsible for FB1 degradation, which eliminated about 90% of 10 μg/mL FB1 in 3 h. Besides, liquid chromatography time-of-flight mass spectrometry (LC-TOF/MS) analysis identified two degradation products of FB1, and their toxicity on the monkey kidney cells (MARC-145) was significantly lower (p < 0.05) compared with the parent FB1. Overall, the consortium SAAS79 and its crude enzymes may be a potential choice for the decontamination of FB1 in the feed and food industry. Also, the bacterial consortium provides a new source of genes for the development of enzymatic detoxification agent.

Keywords

Biodegradation Fumonisin B1 Bacterial consortium Enzymatic detoxification Metabolites 

Notes

Funding

The authors acknowledged the financial support from National Natural Science Foundation of China (31602124, 31871896, and 31401598) and Shanghai Agriculture Commission Basic Research Project (grant number 2014 no. 7-3-7).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2019_9979_MOESM1_ESM.pdf (38 kb)
ESM 1 (PDF 37 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute for Agro-Food Standards and Testing Technology, Laboratory of Quality & Safety Risk Assessment for Agro-Products (Shanghai), Ministry of AgricultureShanghai Academy of Agricultural SciencesShanghaiPeople’s Republic of China
  2. 2.College of Life and ScienceXinyang Normal UniversityXinyangPeople’s Republic of China
  3. 3.CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological SciencesUniversity of Chinese Academy of Sciences, Chinese Academy of SciencesHuairouPeople’s Republic of China

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