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Applied Microbiology and Biotechnology

, Volume 103, Issue 17, pp 7151–7160 | Cite as

Role of 10-hydroxy-cis-12-octadecenic acid in transforming linoleic acid into conjugated linoleic acid by bifidobacteria

  • He Gao
  • Bo Yang
  • Catherine Stanton
  • R. Paul Ross
  • Hao Zhang
  • Haiqin ChenEmail author
  • Wei Chen
Applied microbial and cell physiology

Abstract

10-hydroxy-cis-12 octadecenoic acid (10-HOE) is a type of octadecenoic acid with a hydroxyl on the C10 carbon. It is generated from linoleic acid (LA) catalyzed by linoleate hydratase in lactobacilli, which was initially named as myosin-cross-reactive antigen (MCRA). In lactobacilli, 10-HOE is the first intermediate in the production of conjugated LA (CLA). Although MCRA from bifidobacteria can generate 10-HOE, the precise role of 10-HOE in CLA production in bifidobacteria remains unknown. In the current work, 10-HOE and LA were added to the medium as the substrate both separately and synchronously to analyze their influence on CLA production. Using 10-HOE as the substrate, bifidobacteria were able to generate CLA by first converting it to LA, followed by CLA accumulation. Recombinant MCRA catalyzed the conversion of 10-HOE to LA, indicating that bifidobacterial MCRA can account for the reversible conversion between LA and 10-HOE. This is the first report to demonstrate the precise role of 10-HOE in the process of CLA production among bifidobacteria.

Keywords

Linoleic acid Conjugated linoleic acid 10-Hydroxy-cis-12-octadecenoic acid Bifidobacterium 

Notes

Funding information

This research was funded by the National Natural Science Foundation of China (nos. 31722041, 31801521, 31571810), the Fundamental Research Funds for the Central Universities (nos. JUSRP51702A, JUSRP11733), the national first-class discipline program of Food Science and Technology (JUFSTR20180102), and the Jiangsu Province “Collaborative Innovation Center for Food Safety and Quality Control,” the Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX18_1763).

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxiChina
  2. 2.School of Food Science and TechnologyJiangnan UniversityWuxiChina
  3. 3.International Joint Research Centre for Probiotics & Gut HealthJiangnan UniversityWuxiChina
  4. 4.Teagasc Food Research CentreMoorepark, Fermoy, CoCorkIreland
  5. 5.APC Microbiome IrelandUniversity College CorkCorkIreland
  6. 6.Beijing Innovation Centre of Food Nutrition and Human HealthBeijing Technology & Business UniversityBeijingChina

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