Biosynthetic strategies to produce xylitol: an economical venture
Xylitol is a natural five-carbon sugar alcohol with potential for use in food and pharmaceutical industries owing to its insulin-independent metabolic regulation, tooth rehardening, anti-carcinogenic, and anti-inflammatory, as well as osteoporosis and ear infections preventing activities. Chemical and biosynthetic routes using D-xylose, glucose, or biomass hydrolysate as raw materials can produce xylitol. Among these methods, microbial production of xylitol has received significant attention due to its wide substrate availability, easy to operate, and eco-friendly nature, in contrast with high-energy consuming and environmental-polluting chemical method. Though great advances have been made in recent years for the biosynthesis of xylitol from xylose, glucose, and biomass hydrolysate, and the yield and productivity of xylitol are substantially improved by metabolic engineering and optimizing key metabolic pathway parameters, it is still far away from industrial-scale biosynthesis of xylitol. In contrary, the chemical synthesis of xylitol from xylose remains the dominant route. Economic and highly efficient xylitol biosynthetic strategies from an abundantly available raw material (i.e., glucose) by engineered microorganisms are on the hard way to forwarding. However, synthetic biology appears as a novel and promising approach to develop a super yeast strain for industrial production of xylitol from glucose. After a brief overview of chemical-based xylitol production, we critically analyzed and comprehensively summarized the major metabolic strategies used for the enhanced biosynthesis of xylitol in this review. Towards the end, the study is wrapped up with current challenges, concluding remarks, and future prospects for designing an industrial yeast strain for xylitol biosynthesis from glucose.
KeywordsXylitol Yarrowia lipolytica Biosynthetic routes Metabolic engineering Synthetic biology
This research was supported by the National Natural Science Foundation of China (No. 21877078).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Bon EPS, Ferrara MA, Corvo ML (2008) Enzimas em Biotecnologia - Produção, Aplicação e Mercado (Enzymes in biotechnology: production, application and market). Interciência, Rio de JaneiroGoogle Scholar
- Chun BW, Dair B, Macuch PJ, Wiebe D, Porteneuve C, Jeknavorian A (2006) The development of cement and concrete additive: based on xylonic acid derived via bioconversion of xylose. Appl Microbiol Biotechnol 129-132(1–3):645Google Scholar
- Harkki AM, Myasnikov AN, Apajalahti JHA, Pastinen OA (2004) Manufacture of xylitol using recombinant microbial hosts. United States Patent 6723540Google Scholar
- Hong Y, Dashtban M, Kepka G, Chen S, Qin W (2014b) Overexpression of d-xylose reductase (xyl1) gene and antisense inhibition of dxylulokinase (xyiH) gene increase xylitol production in Trichoderma reesei. Biomed Res Int 2014:8–8Google Scholar
- Jin LQ, Xu W, Yang B, Liu ZQ, Zheng YG (2018) Efficient biosynthesis of xylitol from xylose by coexpression of xylose reductase and glucose dehydrogenase in Escherichia coli. Appl Biochem Biotechnol 187(4), 1143–1157Google Scholar
- Kumar J, Reddy MS, Rao LV (2010) Strain improvement of Candida tropicalis ovc5 for xylitol production by random mutagenesis. IIOAB J 1:24–28Google Scholar
- Lowe DA, Jennings DH (1985) Carbohydrate metabolism in the fungus Dendryphiella salina. V. The pattern of label in arabitol and polysaccharide after growth in the presence of specifically labelled carbon sources. New Phytol 101(4):399–403Google Scholar
- Meng J, Sun W, Wang F, Deng W, Luo Y, Zhu J, Lin J, Wang X, Qi X (2014) Microbial and enzymatic process for xylitol production from D-glucose. Curr Org Chem 18(24):3131–3135Google Scholar
- Nigam P, Singh D (1995) Processes of fermentative production of xylitol—a sugar substitute. Process Biochem 30(2):117–124Google Scholar
- Pepper T, Olinger PM (1988) Xylitol in sugar-free confections. Food Technol 42:98–106Google Scholar
- Suzuki T, Yokoyama S, Kinoshita Y, Yamada H, Hatsu M, Takamizawa K, Kawai K (1999) Expression of xyrA gene encoding for D-xylose reductase of Candida tropicalis and production of xylitol in Escherichia coli. J Ferment Bioeng 87(3):280–284Google Scholar
- Uppada V, Bhaduri S, Noronha SB (2014) Cofactor generation—an important aspect of biocatalysis. Curr Sci 106:946–957Google Scholar
- US Food and Drug Administration (2008) Guidance for industry: a food labeling guide. Food and Drug Administration, Washington, DCGoogle Scholar
- Werpy T, Petersen G, Aden A, Bozell J, Holladay J, White J, Manheim A, Eliot D, Lasure L, Jones S (2004) Top value added chemicals from biomass. Volume 1—results of screening for potential candidates from sugars and synthesis gas. Department of Energy, Washington DCGoogle Scholar
- Yong SK, Sang YK, Kim JH, Sun CK (1999) Xylitol production using recombinant Saccharomyces cerevisiae containing multiple xylose reductase genes at chromosomal δ-sequences. J Biotechnol 67(2–3):159–171Google Scholar
- Yoshitake J, Ishizaki H, Shimamura M, Imai T (2014) Xylitol production by an Enterobacter species. Biosci Biotechnol Biochem 37(10):2261–2267Google Scholar