Bioprospecting microalgae from natural algal bloom for sustainable biomass and biodiesel production

  • Manish K. Pandey
  • Chitralekha Nag Dasgupta
  • Shashank Mishra
  • Manish Srivastava
  • Vijai Kumar Gupta
  • M. R. Suseela
  • P. W. RamtekeEmail author
Bioenergy and biofuels


Natural algal bloom consists of promising algal species which could be a feasible option for the source of bulk biomass and biodiesel production. It has been found in five natural fresh water algal blooms (Uttar Pradesh, India), containing high nitrogen (N) (4.6 ± 0.32 mg/L) and phosphorus (P) (4.12 ± 0.29 mg/L) concentration during spring (23.9–25.9 °C) and summer season (32.0–35.0 °C). Among the isolated algae from naturally occurring bloom, Chlorella sorokiniana MKP01 exhibited highest biomass (1.02 ± 0.02 g/L) and lipid content (174.1 ± 9.6 mg/L) in untreated tap water and urea/single super phosphate (SSP) in the ratio (2:1). The biodiesel quality was assessed and found to be with the Indian and international standards. Algal bloom was artificially developed in the open pond containing 10,000 l tap water supplemented with Urea/SSP (2:1) for a consistent supply of bulk biomass, yielded 8 kg of total biomass and lipid 1.3 kg.


Algal bloom Water parameters Agricultural fertilizer Biomass  Biofuels 



The authors are thankful to the Director, CSIR-National Botanical Research Institute, Lucknow for the constant encouragement and necessary laboratory facilities. The authors acknowledge all lab members for their support.

Funding information

The Department of Biotechnology (DBT), Delhi, India provided financial support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any experiments involving human participants or animals performed by any of the authors.

Supplementary material

253_2019_9856_MOESM1_ESM.pdf (428 kb)
ESM 1 (PDF 428 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Biological sciencesSam Higginbottom University of Agriculture Technology and SciencesAllahabadIndia
  2. 2.Algology LaboratoryCSIR-National Botanical Research InstituteLucknowIndia
  3. 3.Quality Control and Quality Analysis LaboratoryLucknowIndia
  4. 4.Department of Physics and AstrophysicsUniversity of DelhiDelhiIndia
  5. 5.Department of Chemistry and Biotechnology, School of ScienceTallinn University of TechnologyTallinEstonia

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