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Applied Microbiology and Biotechnology

, Volume 103, Issue 14, pp 5797–5809 | Cite as

Complete mitogenome of the entomopathogenic fungus Sporothrix insectorum RCEF 264 and comparative mitogenomics in Ophiostomatales

  • Shu ZhangEmail author
  • Yong-Jie ZhangEmail author
  • Zhi-Liang Li
Genomics, transcriptomics, proteomics

Abstract

The fungal order Ophiostomatales contains numerous species important in medical fields, agriculture, and forestry, and several species have had available mitogenome information. The nuclear genome of the entomopathogenic fungus Sporothrix insectorum has been reported, while its mitogenome remains unknown. Herein, we firstly described the mitogenome of S. insectorum RCEF 264 and then compared Ophiostomatales mitogenomes from both interspecific and intraspecific perspectives. The mitogenome of S. insectorum RCEF 264 was 31,454 bp in length, containing typical fungal mitochondrial genes plus rnpB. Four group I introns interrupted rnl and cox1. Phylogenetic analyses confirmed the placement of S. insectorum RCEF 264 in Ophiostomatales. Comparison of mitogenomes among seven Ophiostomatales species revealed conserved gene contents and a high synteny, although there were also some differences among them. Their mitogenomes showed more than two-fold variations (26.6–65.1 kb) in size, with a total of 37 intron insertional loci from 11 genes (1–25 introns per species). The sole intron shared by all species was an rps3-encoding intron in rnl (mL2450), and this intron-based phylogeny was highly consistent with those constructed using mitochondrial/nuclear genes, suggesting convergent evolution of this intron with Ophiostomatales species. The dendrogram based on presence/absence patterns at all intron loci was quite different from those based on mitochondrial/nuclear genes. Comparison of mitogenomes among two to three intraspecific individuals in Ophiostoma novo-ulmi subsp. novo-ulmi and Sporothrix schenckii revealed mitogenome size variations due to single-nucleotide polymorphisms (SNPs) and indels but without fluctuation of intron numbers for each species. This study greatly enhanced our understanding of mitogenome evolution in Ophiostomatales.

Keywords

Sporothrix insectorum Ophiostomatales Mitogenome Intron Phylogeny Evolution Comparative mitogenomics 

Notes

Acknowledgments

The authors thank Prof. Cheng-Shu Wang at Institute of Plant Physiology and Ecology, Chinese Academy of Sciences for providing the S. insectorum strain. We also thank Mr. Ning-Bo Cui for helping amplify R1295_mt and R1295_nr.

Funding information

This study was funded by the National Natural Science Foundation of China (31872162), the Natural Science Foundation of Shanxi Province (201601D011065), the Fund Program for the Scientific Activities of Selected Returned Overseas Professionals in Shanxi Province, the Research Project Supported by Shanxi Scholarship Council of China (2017-015), Hundred Talents Program of Shanxi Province, and the Special Fund for Large Scientific Instruments and Equipment in Shanxi Province.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2019_9855_MOESM1_ESM.pdf (576 kb)
ESM 1 (PDF 575 kb)
253_2019_9855_MOESM2_ESM.xlsx (60 kb)
ESM 2 (XLSX 59 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Applied ChemistryShanxi UniversityTaiyuanChina
  2. 2.School of Life ScienceShanxi UniversityTaiyuanChina
  3. 3.School of AgricultureLudong UniversityYantaiChina

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