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Roles of three TonB systems in the iron utilization and virulence of the Aeromonas hydrophila Chinese epidemic strain NJ-35

  • Yuhao Dong
  • Jinzhu Geng
  • Jin Liu
  • Maoda Pang
  • Furqan Awan
  • Chengping Lu
  • Yongjie LiuEmail author
Applied microbial and cell physiology
  • 65 Downloads

Abstract

The TonB system functions in iron transport and has been identified in certain Gram-negative bacteria. Recently, we reported three TonB systems in the Aeromonas hydrophila Chinese epidemic strain NJ-35, but the functions of these systems have not been thoroughly elucidated to date. In this study, we investigated the role of these TonB systems in A. hydrophila iron utilization and virulence. We found that tonB1 and tonB2 were preferentially transcribed in iron-chelated conditions, where gene expression levels were approximately 8- and 68-fold higher compared with iron-rich conditions, respectively; tonB3 was consistently transcribed at a low level under iron-repleted and iron-depleted conditions. Only the TonB2 system was required to utilize iron-binding proteins. The tonB123 mutant showed increased susceptibility to erythromycin and roxithromycin. In addition, all three tonB genes were involved in A. hydrophila virulence in zebrafish, and various phenotypes associated with environmental survival were changed with varying degrees in each tonB mutant. TonB2 plays a relatively major role in adhesion, motility, and biofilm formation, while TonB3 is more involved in the anti-phagocytosis of A. hydrophila. In each observed phenotype, no significant difference was found between the single- and double-deletion mutants, whereas the triple-deletion mutant exhibited the most serious defects, indicating that all three TonB systems of A. hydrophila coordinately complement one another. In conclusion, this study elucidates the importance of TonB in iron acquisition and virulence of A. hydrophila, which lays the foundation for future studies regarding the survival mechanisms of this bacterium in iron-restricted environments.

Keywords

Aeromonas hydrophila TonB systems Iron acquisition Virulence Environmental survival 

Notes

Funding information

This work was supported by the National Natural Science Foundation of China (31372454), the Three New Aquatic Projects in Jiangsu Province (D2017-3-1), the Independent Innovation Fund of Agricultural Science and Technology in Jiangsu Province (CX(17)2027), and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Experiments involving live animals were carried out in accordance with animal welfare standards and were approved by the Ethical Committee for Animal Experiments of Nanjing Agricultural University, China. All experiments involving animals complied with the guidelines of the Animal Welfare Council of China.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Yuhao Dong
    • 1
  • Jinzhu Geng
    • 1
  • Jin Liu
    • 1
  • Maoda Pang
    • 2
  • Furqan Awan
    • 1
  • Chengping Lu
    • 1
  • Yongjie Liu
    • 1
    Email author
  1. 1.Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary MedicineNanjing Agricultural UniversityNanjingChina
  2. 2.Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Safety and NutritionJiangsu Academy of Agricultural SciencesNanjingChina

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