Oleaginous yeasts for sustainable lipid production—from biodiesel to surf boards, a wide range of “green” applications
A growing world population and a growing number of applications for vegetable oils are generating an increasing demand for these oils, causing serious environmental problems. A sustainable lipid production is then fundamental to address these problems. Oleaginous yeasts are a promising solution for sustainable lipid production, but, with the current knowledge and technology, they are still not a serious alternative in the market. In this review, the potential of these yeasts is highlighted and a discussion is made mainly focused on the economics of the oleaginous yeast oil production and identification of the key points to be improved to achieve lower production costs and higher income. Three main stages of the production process, where costs are higher, were identified. To render economically feasible the production of oils using oleaginous yeasts, a reduction in production costs must occur in all stages, lipid yields and productivities must be improved, and production must be targeted to high-value product applications.
KeywordsOleaginous yeasts Vegetable oils Sustainability Biodiesel Lipids
This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), FCT doctoral grant (SFRH/BD/80490/2011) attributed to Bruno Vasconcelos, and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte.
Compliance with ethical standards
Conflict of interest
Bruno Vasconcelos declares that he has no conflict of interest. José Carlos Teixeira declares that he has no conflict of interest. Giuliano Dragone declares that he has no conflict of interest. José António Teixeira declares that he has no conflict of interest.
Human and animal studies
This article does not contain any studies with human participants or animals performed by any of the authors.
- Alvarez RM, Rodriguez B, Romano JM, Diaz AO, Mir D, Navarro L, Saura G, Garcia JL (1992) Lipid accumulation in Rhodotorula glutinis on sugar cane molasses in single-stage continuous culture. J Microbiol 8:214–215Google Scholar
- Ca V, Lligadas G, Ronda JC, Galia M (2013) Renewable polymeric materials from vegetable oils: a perspective. 16:337–343. https://doi.org/10.1016/j.mattod.2013.08.016
- Dai C, Tao J, Xie F, Dai Y, Zhao M (2007) Biodiesel generation from oleaginous yeast Rhodotorula glutinis with xylose assimilating capacity. J Biotechnol 6:2130–2134Google Scholar
- Fei Q, O’Brien M, Nelson R, Chen X, Lowell A, Dowe N (2016) Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source. Biotechnol Biofuels 9:1–12. https://doi.org/10.1186/s13068-016-0542-x Google Scholar
- Hassan M, Blanc PJ, Pareilleux A, Goma G (1994) Production of single-cell oil from prickly- pear juice fermentation by Cryptococcus curvatus grown in batch culture. Microbiology 1:1994–1994Google Scholar
- Kot AM, Kurcz A, Bry J, Gientka I, Bzducha-wróbel A, Maliszewska M, Reczek L (2017) Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis. 27:25–31. https://doi.org/10.1016/j.ejbt.2017.01.007
- Marelli L, Padella M, Edwards R, Moro A, Kousoulidou M, Giuntoli J, Baxter D, Vorkapic V, O’Connell A, Lonza L (2015) The impact of biofuels on transport and the environment, and their connection with agricultural development in EuropeGoogle Scholar
- Mata-Gómez LC, Montañez JC, Méndez-zavala A, Aguilar CN (2018) Biotechnological production of carotenoids by yeasts: an overview. 1–11Google Scholar
- Ochsenreither K, Glück C, Stressler T, Fischer L, Syldatk C (2016) Production strategies and applications of microbial single cell oils. Front Microbiol 7. https://doi.org/10.3389/fmicb.2016.01539
- Pan JG, Kwak MY, Rhee JS (1986) High density cell-culture of Rhodtorula Glutinis using oxygen-enriched air. Biotechnol Lett 8:715–718Google Scholar
- Papanikolaou S, Aggelis G (2002) Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single-stage continuous culture. Bioresour Technol 82:43–49Google Scholar
- Probst KV, Schulte LR, Durrett TP, Rezac ME, Probst KV, Schulte LR, Durrett TP, Rezac ME, Probst KV, Schulte LR, Durrett TP, Rezac ME, Vadlani PV (2017) Oleaginous yeast: a value-added platform for renewable oils. 8551. https://doi.org/10.3109/07388551.2015.1064855
- Ratledge C (2002) Regulation of lipid accumulation in oleaginous micro-organisms. Biochem Soc Trans 30:1047–1050Google Scholar
- Ricardo C, José C, Neto D, Thomaz V, Bittencourt E, Scopel E, Bianchi A, Medeiros P, Porto L, Vandenberghe DS (2017) Pilot scale biodiesel production from microbial oil of Rhodosporidium toruloides DEBB 5533 using sugarcane juice : performance in diesel engine and preliminary economic study. Bioresour Technol 223:259–268. https://doi.org/10.1016/j.biortech.2016.10.055 Google Scholar
- Saenge C, Cheirsilp B, Tachapattaweawrakul T, Bourtoom T, Suksaroge TT (2011) Potential use of oleaginous red yeast Rhodotorula glutinis for the bioconversion of crude glycerol from biodiesel plant to lipids and carotenoids. Process Biochem 46:210–218. https://doi.org/10.1016/j.procbio.2010.08.009 Google Scholar
- Sargeant LA, Chuck CJ, Donnelly J, Bannister CD, Scott RJ (2014) Optimizing the lipid profile, to produce either a palm oil or biodiesel substitute, by manipulation of the culture conditions for Rhodotorula glutinis. 5:33–43Google Scholar
- Sitepu IR, Sestric R, Ignatia L, Levin D, German JB, Gillies LA, Almada LAG, Boundy-Mills KL (2013) Manipulation of culture conditions alters lipid content and fatty acid profiles of a wide variety of known and new oleaginous yeast species. Bioresour Technol 144:360–369. https://doi.org/10.1016/j.biortech.2013.06.047 Google Scholar
- Wild R, Patil S, Popovi M, Zappi M, Dufreche S, Bajpai R (2010) Lipids from Lipomyces starkeyi. Wild 48:329–335Google Scholar
- Yen H-W, Yang Y-C, Yu Y-H (2012) Using crude glycerol and thin stillage for the production of microbial lipids through the cultivation of Rhodotorula glutinis. J Biosci Bioeng xx. https://doi.org/10.1016/j.jbiosc.2012.04.022
- Ykema A, Verbree EC, Kater MM, Smit H (1988) Optimization of lipid production in the oleaginous yeast Apiotrichum curvature in whey permeate. Growth (Lakeland) 211–218Google Scholar
- Zhang C, Garrison TF, Madbouly SA, Kessler MR (2017) Recent advances in vegetable oil-based polymers and their composites. Prog Polym Sci 71:91–143. https://doi.org/10.1016/J.PROGPOLYMSCI.2016.12.009 Google Scholar