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Applied Microbiology and Biotechnology

, Volume 103, Issue 7, pp 3099–3109 | Cite as

New two-component regulatory system required for the constitutive expression of bph operon in Cupriavidus basilensis WS

  • Sheng Wang
  • Yajun Li
  • Bing Wang
  • Linna Du
  • Hui Jiang
  • Yuhua ZhaoEmail author
Applied genetics and molecular biotechnology
  • 156 Downloads

Abstract

Cupriavidus basilensis WS degrades diphenyl ether (DE) and its lower brominated derivatives using enzymes encoded by the bph operon. However, it is not yet known under what circumstances bph genes are expressed and how they are regulated in C. basilensis WS. To answer these questions, we used transposon mutagenesis and identified a new two-component regulatory system, BphS/BphT, in C. basilensis WS, which is indispensable for the expression of the bph operon. When BphS or BphT is inactivated, C. basilensis WS no longer exhibits the ability to decompose DE. Using a β-galactosidase reporter system and RT-qPCR, we showed that bph genes are constitutively transcribed in C. basilensis WS and that deletion of bphS or bphT strongly inhibited the transcription of bph genes. We also showed that the gene ORF0, which is upstream of bphA1 and is similar to the GntR-family regulators of the bph operon, is not involved in the constitutive transcription of the bph operon in C. basilensis WS. The cis-acting elements required for the expression and regulation of bph genes in the DE degradation pathway are included in the intergenic region between ORF0 and bphA1. Our results suggest that BphS/BphT represents a new two-component regulatory system for the bph operon that is necessary for the constitutive expression of bph genes.

Keywords

bph operon Transcription Two-component regulatory system Diphenyl ether 

Notes

Funding information

This study was supported by the National Key Basic Research Program of China (2015CB150502), the National Natural Science Foundation of China (31470191, 41671314, 41877114), the Zhejiang Provincial Natural Science Foundation of China (LQ17D010001), and the Key Research and Development Program of Zhejiang Province (2015C03011).

Compliance with ethical standards

Conflict of interest

The authors declare they have no conflict of interest.

Human and animal rights and informed consent

This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

253_2019_9686_MOESM1_ESM.pdf (960 kb)
ESM 1 (PDF 959 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Sheng Wang
    • 1
    • 2
  • Yajun Li
    • 1
  • Bing Wang
    • 3
  • Linna Du
    • 2
  • Hui Jiang
    • 1
  • Yuhua Zhao
    • 1
    Email author
  1. 1.College of Life SciencesZhejiang UniversityZhejiangPeople’s Republic of China
  2. 2.Wenzhou Vocational College of Science & TechnologyZhejiangPeople’s Republic of China
  3. 3.Hangzhou Center for Disease Control and PreventionZhejiangPeople’s Republic of China

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