Development and validation of a competitive ELISA based on bacterium-original virus-like particles of serotype O foot-and-mouth disease virus for detecting serum antibodies
- 214 Downloads
Foot-and-mouth disease (FMD) is a highly contagious disease that affects all susceptible cloven-hoofed animals, resulting in considerable economic losses to animal industries worldwide. Numerous categories of enzyme-linked immunosorbent assays (ELISA) have been developed and widely used to evaluate herd immunity. Manufacturing inactivated FMD virus (FMDV) as a diagnostic antigen requires a facility with a high level of biosafety, but this requirement raises concern on viral leakage. In our previous study, bacterium-original FMD virus-like particles (VLPs) resemble the authentic FMDV and induce protective immunity against homologous viral challenges, thereby demonstrating that they are sufficiently safe without limitations on biosafety facilities and easily prepared. Herein, we developed a competitive ELISA (cELISA) based on FMDV-VLPs as a diagnostic antigen to evaluate herd immunity. The criterion of this cELISA was determined by detecting panels of positive sera with different antibody titers and negative sera. The working parameter of cELISA was optimized, and samples with a percentage inhibition of ≥ 50% were considered positive. The specificity of cELISA to test 277 serum samples with various antibody titers was 100%, and the sensitivity reached 96%. The coincidence rates of cELISA with a VDPro® FMDV and a PrioCHECK® FMDV type O antibody ELISA kit were 97.8% and 98.2%, respectively. Repeatability tests demonstrated that the coefficients of variation within and between runs were less than 7% and 14%, respectively. Our data demonstrated that cELISA based on bacterium-original VLPs had high specificity, sensitivity, and reproducibility. The cELISA could also be used for evaluating vaccination herd immunity effects, especially in developing countries.
KeywordsFoot-and-mouth disease Competitive ELISA Virus-like particles Herd immunity Validation
XR contributed to the preparation of the manuscript. ZY, HG, and MB designed this analysis. ZY conducted the experiment. XR, XW, and ZY reviewed records. ZY, XR, HG, and SS analyzed the data. ZY and SS prepared the materials. All authors reviewed the manuscript and approved the final manuscript.
This study was supported by the grants “the National Key Research and Development Program of China (2017YFD0501100, 2016YFD05007000)” and the Graduate student innovation fund of Heilongjiang Bayi Agricultural University (YJSCX2017-Y43).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All of the animal experiments were conducted in accordance with the regulations for the administration of affairs concerning experimental animals approved by the State Science and Technology Commission of the People’s Republic of China.
- Abubakar M, Manzoor S, Ahmed A (2018) Interplay of foot and mouth disease virus with cell-mediated and humoral immunity of host. Rev Med Virol 28(2). https://doi.org/10.1002/rmv.1966
- Basagoudanavar SH, Hosamani M, Tamil Selvan RP, Sreenivasa BP, Saravanan P, Chandrasekhar Sagar BK, Venkataramanan R (2013) Development of a liquid-phase blocking ELISA based on foot-and-mouth disease virus empty capsid antigen for seromonitoring vaccinated animals. Arch Virol 158(5):993–1001. https://doi.org/10.1007/s00705-012-1567-5 CrossRefPubMedGoogle Scholar
- Chung CJ, Clavijo A, Bounpheng MA, Uddowla S, Sayed A, Dancho B, Olesen IC, Pacheco J, Kamicker BJ, Brake DA, Bandaranayaka-Mudiyanselage CL, Lee SS, Rai DK, Rieder E (2018) An improved, rapid competitive ELISA using a novel conserved 3B epitope for the detection of serum antibodies to foot-and-mouth disease virus. J Vet Diagn Investig 1040638718779641:699–707. https://doi.org/10.1177/1040638718779641 CrossRefGoogle Scholar
- Feng X, Ma JW, Sun SQ, Guo HC, Yang YM, Jin Y, Zhou GQ, He JJ, Guo JH, Qi SY, Lin M, Cai H, Liu XT (2016) Quantitative detection of the foot-and-mouth disease virus serotype O 146S antigen for vaccine production using a double-antibody sandwich ELISA and nonlinear standard curves. PLoS One 11(3):e0149569. https://doi.org/10.1371/journal.pone.0149569 CrossRefPubMedGoogle Scholar
- Guo HC, Sun SQ, Jin Y, Yang SL, Wei YQ, Sun DH, Yin SH, Ma JW, Liu ZX, Guo JH, Luo JX, Yin H, Liu XT, Liu DX (2013) Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in Escherichia coli induce potent protective immune responses in guinea pigs, swine and cattle. Vet Res 44:48. https://doi.org/10.1186/1297-9716-44-48 CrossRefPubMedGoogle Scholar
- Hosamani M, Basagoudanavar SH, Tamil Selvan RP, Das V, Ngangom P, Sreenivasa BP, Hegde R, Venkataramanan R (2015) A multi-species indirect ELISA for detection of non-structural protein 3ABC specific antibodies to foot-and-mouth disease virus. Arch Virol 160(4):937–944. https://doi.org/10.1007/s00705-015-2339-9 CrossRefPubMedGoogle Scholar
- Jaworski JP, Compaired D, Trotta M, Perez M, Trono K, Fondevila N (2011) Validation of an r3AB1-FMDV-NSP ELISA to distinguish between cattle infected and vaccinated with foot-and-mouth disease virus. J Virol Methods 178(1–2):191–200. https://doi.org/10.1016/j.jviromet.2011.09.011 CrossRefPubMedGoogle Scholar
- Ko YJ, Jeoung HY, Lee HS, Chang BS, Hong SM, Heo EJ, Lee KN, Joo HD, Kim SM, Park JH, Kweon CH (2009) A recombinant protein-based ELISA for detecting antibodies to foot-and-mouth disease virus serotype Asia 1. J Virol Methods 159(1):112–118. https://doi.org/10.1016/j.jviromet.2009.03.011 CrossRefPubMedGoogle Scholar
- Ko YJ, Lee HS, Park JH, Lee KN, Kim SM, Cho IS, Joo HD, Paik SG, Paton DJ, Parida S (2012) Field application of a recombinant protein-based ELISA during the 2010 outbreak of foot-and-mouth disease type A in South Korea. J Virol Methods 179(1):265–268. https://doi.org/10.1016/j.jviromet.2011.09.021 CrossRefPubMedGoogle Scholar
- Lu Z, Cao Y, Guo J, Qi S, Li D, Zhang Q, Ma J, Chang H, Liu Z, Liu X, Xie Q (2007) Development and validation of a 3ABC indirect ELISA for differentiation of foot-and-mouth disease virus infected from vaccinated animals. Vet Microbiol 125(1–2):157–169. https://doi.org/10.1016/j.vetmic.2007.05.017 CrossRefPubMedGoogle Scholar
- Mahapatra M, Yuvaraj S, Madhanmohan M, Subramaniam S, Pattnaik B, Paton DJ, Srinivasan VA, Parida S (2015) Antigenic and genetic comparison of foot-and-mouth disease virus serotype O Indian vaccine strain, O/IND/R2/75 against currently circulating viruses. Vaccine 33(5):693–700. https://doi.org/10.1016/j.vaccine.2014.11.058 CrossRefPubMedGoogle Scholar
- Oem JK, Park JH, Lee KN, Kim YJ, Kye SJ, Park JY, Song HJ (2007) Characterization of recombinant foot-and-mouth disease virus pentamer-like structures expressed by baculovirus and their use as diagnostic antigens in a blocking ELISA. Vaccine 25(20):4112–4121. https://doi.org/10.1016/j.vaccine.2006.08.046 CrossRefPubMedGoogle Scholar
- OIE, World Organization for Animal Health (2017) Foot and mouth disease (infection with food and mouth disease virus). Chapter 2.1.8. Available online: http://220.127.116.11/www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.01.08_FMD.pdf. Accessed Feb 15 2019
- Paiba GA, Anderson J, Paton DJ, Soldan AW, Alexandersen S, Corteyn M, Wilsden G, Hamblin P, MacKay DK, Donaldson AI (2004) Validation of a foot-and-mouth disease antibody screening solid-phase competition ELISA (SPCE). J Virol Methods 115(2):145–158. https://doi.org/10.1016/j.jviromet.2003.09.016 CrossRefPubMedGoogle Scholar
- Porphyre T, Rich KM, Auty HK (2018) Assessing the economic impact of vaccine availability when controlling foot and mouth disease outbreaks. Front Vet Sci 5(47). https://doi.org/10.3389/fvets.2018.00047
- Rawdon TG, Garner MG, Sanson RL, Stevenson MA, Cook C, Birch C, Roche SE, Patyk KA, Forde-Folle KN, Dube C, Smylie T, Yu ZD (2018) Evaluating vaccination strategies to control foot-and-mouth disease: a country comparison study. Epidemiol Infect 1–13. https://doi.org/10.1017/S0950268818001243
- Roche SE, Garner MG, Sanson RL, Cook C, Birch C, Backer JA, Dube C, Patyk KA, Stevenson MA, Yu ZD, Rawdon TG, Gauntlett F (2015) Evaluating vaccination strategies to control foot-and-mouth disease: a model comparison study. Epidemiol Infect 143(6):1256–1275. https://doi.org/10.1017/S0950268814001927 CrossRefPubMedGoogle Scholar
- Sharma GK, Mohapatra JK, Pandey LK, Mahajan S, Mathapati BS, Sanyal A, Pattnaik B (2012) Immunodiagnosis of foot-and-mouth disease using mutated recombinant 3ABC polyprotein in a competitive ELISA. J Virol Methods 185(1):52–60. https://doi.org/10.1016/j.jviromet.2012.05.029 CrossRefPubMedGoogle Scholar
- Xiao Y, Chen HY, Wang Y, Yin B, Lv C, Mo X, Yan H, Xuan Y, Huang Y, Pang W, Li X, Yuan YA, Tian K (2016) Large-scale production of foot-and-mouth disease virus (serotype Asia1) VLP vaccine in Escherichia coli and protection potency evaluation in cattle. BMC Biotechnol 16(1):56. https://doi.org/10.1186/s12896-016-0285-6 CrossRefPubMedGoogle Scholar
- Yang M, Parida S, Salo T, Hole K, Velazquez-Salinas L, Clavijo A (2015) Development of a competitive enzyme-linked immunosorbent assay for detection of antibodies against the 3B protein of foot-and-mouth disease virus. Clin Vaccine Immunol 22(4):389–397. https://doi.org/10.1128/CVI.00594-14 CrossRefPubMedGoogle Scholar