Applied Microbiology and Biotechnology

, Volume 103, Issue 7, pp 2973–2984 | Cite as

Bioengineering of microbial transglutaminase for biomedical applications

  • Soo Khim ChanEmail author
  • Theam Soon Lim


Microbial transglutaminase (mTGase) is commonly known in the food industry as meat glue due to its incredible ability to “glue” meat proteins together. Aside from being widely exploited in the meat processing industries, mTGase is also widely applied in other food and textile industries by catalysing the formation of isopeptide bonds between peptides or protein substrates. The advancement of technology has opened up new avenues for mTGase in the field of biomedical engineering. Efforts have been made to study the structural properties of mTGase in order to gain an in-depth understanding of the structure-function relationship. This review highlights the developments in mTGase engineering together with its role in biomedical applications including biomaterial fabrication for tissue engineering and biotherapeutics.


Microbial transglutaminase Gelatin Chitosan Collagen Biomaterials Antibody-drug conjugate 



This study was funded by Malaysian Ministry of Education under the Higher Institutions Centre of Excellence (HICoE) Grant Scheme (311/CIPPM/4401005).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute for Research in Molecular MedicineUniversiti Sains MalaysiaPenangMalaysia
  2. 2.Analytical Biochemistry Research CentreUniversiti Sains MalaysiaPenangMalaysia

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