Applied Microbiology and Biotechnology

, Volume 103, Issue 5, pp 2101–2111 | Cite as

Strategy for improving L-isoleucine production efficiency in Corynebacterium glutamicum

  • Xiaoyuan WangEmail author


As one of the three branched-chain amino acids essential for human body, L-isoleucine is widely used in food, medicine, and feed industries. At present, L-isoleucine is mainly produced by microbial fermentation, and the main production strain is Corynebacterium glutamicum. The biosynthetic pathway of L-isoleucine in C. glutamicum is complex, and the activity of key enzymes and the transcription of key genes in the pathway are strictly regulated. The intracellularly synthesized L-isoleucine is secreted by transporters, and the activity of the transporters is also regulated. These intricate regulatory mechanisms increase the difficulty to engineer the L-isoleucine-producing C. glutamicum. This article focuses on the mechanism of L-isoleucine biosynthesis, secretion, and regulation in C. glutamicum and reviews the various metabolic engineering strategies for improving L-isoleucine production efficiency in C. glutamicum.


Corynebacterium glutamicum L-Isoleucine Metabolic engineering Branched-chain amino acids Microbial fermentation L-Isoleucine biosynthesis Metabolic regulation Strain development 



This work was supported by the National First-class Discipline Program of Light Industry Technology and Engineering (LITE2018-10), and the Collaborative Innovation Center of Jiangsu Modern Industrial Fermentation.

Compliance with ethical standards

This article does not contain any studies with human or animal subject.

Conflict of interest

The author declares that he has no conflict of interest.


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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxiChina
  2. 2.Key Laboratory of Industrial Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina

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