Salinity-driven heterogeneity toward anammox distribution and growth kinetics
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Anaerobic ammonium oxidation (anammox) has been widely applied for biological nitrogen removal in freshwater systems, and there is a potential for its extension in saline water systems. In this study, the abundance and biodiversity of anammox bacteria were investigated in both saline and freshwater full-scale sewage treatment plants (STPs). The anammox bacteria were widely found in four tested STPs with abundance of 105–107 copies per mL of 16S rRNA gene. Phylogenetic results showed that Ca. Scalindua and Ca. Brocadia dominated in saline and freshwater STPs, respectively. Ca. Kuenenia dominated in one of freshwater STPs. However, redundancy discriminate analysis (RDA) indicates the distribution of Ca. Kuenenia in both saline and freshwater conditions. To further elucidate these observations, the Monod model was integrated with Gauss equation for the evaluation of salinity-induced kinetics. Model results reveal that when nitrite concentration (SNO2−) is higher than nitrite affinity constant (KNO2−), salinity (over ~ 3.0%) is responsible for Candidatus Scalindua dominance over Candidatus Kuenenia. Conversely, in nitrite-depleted conditions (KNO2− ≥ SNO2−), high nitrite affinity leads to the predominance of Ca. Scalindua in all salinities. This study provides fundamental insights into saline anammox applications.
KeywordsAnammox Salinity Ca. Scalindua Ca. Kuenenia Kinetic modeling
This study received funds from the Research Grants Council (RGC) General Research Fund (15273316), Collaborative Research Fund (C7044-14G), Theme-based Fund (T21-711/16-R), and grant (1-ZVJU) from the Hong Kong Polytechnic University.
Compliance with ethical standards
Human and animal rights and informed consent
This paper does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
The authors declare that they have no conflict of interest.
- Bastian M, Heymann S, Jacomy M (2009) Gephi: an open source software for exploring and manipulating networks. Icwsm 8:361–362Google Scholar
- Hall TA(1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic acids symposium series 41(41), Information Retrieval Ltd.,c1979-c2000, London., p 95–98Google Scholar
- Ji X, Huang Y-T, Wang Q, Tan GYA, Lin J-G, Lee P-H (2015) State-of-the-Art anaerobic ammonium oxidation (anammox) technology. In: Fang, HHP and Zhang T (eds) Anaerobic biotechnology: environmental protection and resource recovery. World Scientific, pp 49–71Google Scholar
- Juretschko S, Timmermann G, Schmid M, Schleifer K-H, Pommerening-Röser A, Koops H-P, Wagner M (1998) Combined molecular and conventional analyses of nitrifying bacterium diversity in activated sludge: Nitrosococcus mobilis and Nitrospira-like bacteria as dominant populations. Appl Environ Microbiol 64(8):3042–3051PubMedPubMedCentralGoogle Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425Google Scholar
- Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75(23):7537–7541CrossRefGoogle Scholar
- Schmid M, Walsh K, Webb R, Rijpstra WI, van de Pas-Schoonen K, Verbruggen MJ, Hill T, Moffett B, Fuerst J, Schouten S (2003) Candidatus “Scalindua brodae”, sp. nov., Candidatus “Scalindua wagneri”, sp. nov., two new species of anaerobic ammonium oxidizing bacteria. Syst Appl Microbiol 26(4):529–538CrossRefGoogle Scholar
- Ter Braak CJ, Smilauer P (2002) CANOCO reference manual and CanoDraw for Windows user's guide: software for canonical community ordination (version 4.5). www. canoco. comGoogle Scholar