Applied Microbiology and Biotechnology

, Volume 103, Issue 2, pp 917–927 | Cite as

In vitro antioxidant activities of Rhodobacter sphaeroides and protective effect on Caco-2 cell line model

  • Jun An
  • Cui Yang
  • Zuming LiEmail author
  • Patricia W. Finn
  • David L. Perkins
  • Jun Sun
  • Zhihui Bai
  • Liping Gao
  • Michael Zhang
  • Difeng Ren
Applied microbial and cell physiology


The present study aimed to evaluate the in vitro antioxidant activities and the protective effect of Rhodobacter sphaeroides on H2O2-induced oxidative stress in Caco-2 cells. The results showed that the antioxidant action of R. sphaeroides varied with different cell concentrations and treatments. Also, the intact cells and intracellular cell-free extracts showed better antioxidant activities. Caco-2 cell–based oxidative stress model was developed by optimizing H2O2 concentration and culture time with the half lethal dose and methyl thiazolyl tetrazolium. By increasing the activity of endogenous antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, upregulating the antioxidant ability of the anti-superoxide anion and anti-hydroxyl radical, R. sphaeroides, especially the mutant strain R. sphaeroides (CGMCC No. 8513), exhibited significant protective activity against H2O2-induced oxidative stress in Caco-2 cells. Taken together, R. sphaeroides (CGMCC No. 8513) exhibits strong antioxidant activities and is a candidate to be investigated as a potential probiotic in the future.


Rhodobacter sphaeroides Antioxidant activities Oxidative stress Caco-2 cell 


Funding information

This work was supported by the China Scholarship Council Foundation (Grant Number 201708110129), the Beijing Natural Science Foundation (Grant Number 6173033), the National Natural Science Foundation of China (Grant Number 31570494), and the Beijing Municipal Commission of Education (Grant Numbers KM20131141 7007 and PXM2013_ 014209_07_ 000082). We also gratefully acknowledge the financial support of the Scientific Research Project from Facing Characteristic Discipline of Beijing Union University.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jun An
    • 1
    • 2
  • Cui Yang
    • 1
  • Zuming Li
    • 1
    Email author
  • Patricia W. Finn
    • 3
  • David L. Perkins
    • 3
  • Jun Sun
    • 3
  • Zhihui Bai
    • 4
  • Liping Gao
    • 1
  • Michael Zhang
    • 5
  • Difeng Ren
    • 2
  1. 1.Department of Food Science and Beijing Key Laboratory of Bioactive Substances and Functional Foods, College of Biochemical EngineeringBeijing Union UniversityBeijingChina
  2. 2.Beijing Key Laboratory of Forest Food Processing and Safety, College of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijingChina
  3. 3.Department of MedicineUniversity of Illinois at ChicagoChicagoUSA
  4. 4.Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina
  5. 5.Department of Physics and AstronomyUniversity of ManitobaWinnipegCanada

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