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Applied Microbiology and Biotechnology

, Volume 103, Issue 2, pp 695–705 | Cite as

Conversion of wastewater-originated waste grease to polyunsaturated fatty acid-rich algae with phagotrophic capability

  • Suo Xiao
  • Lu-Kwang JuEmail author
Biotechnological products and process engineering
  • 128 Downloads

Abstract

Grease balls collected from a municipal wastewater treatment plant were melt-screened and used for cultivation of microalga Ochromonas danica, which could phagocytize droplets and particles as food. After autoclaving, the waste grease (WG) separated into two (upper and lower) phases. O. danica grew well on both, accumulating 48–79% (w/w) intracellular lipids. Initial WG contained approximately 50:50 triglycerides and free fatty acids (FFAs); over time, almost only FFAs remained in the extracellular WG presumably due to hydrolysis by algal lipase. PUFAs, mainly C18:2n6, C18:3n3, C18:3n6, C20:4n6, and C22:5n6, were synthesized and enriched to up to 67% of intracellular FAs, from the original 15% PUFA content in WG. The study showed feasibility of converting wastewater-originated WG to PUFA-rich O. danica algae culture, possibly as aquaculture/animal feed. WG dispersion was identified as a major processing factor to further improve for optimal WG conversion rate and cell and FA yields.

Keywords

Waste grease Wastewater treatment plant Phagotrophic algae Polyunsaturated fatty acids Sustainability 

Notes

Acknowledgments

The authors thank Mr. Gilbert Stadler of the Akron Water Reclamation Facility (Akron, OH) for assistance in grease ball sample collection. The authors also acknowledge the assistance of Dr. Nicholas Callow and Mr. Jacob Kohl in fatty acid analysis.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Abomohra AE-F, El-Sheekh M, Hanelt D (2014) Extracellular secretion of free fatty acids by the chrysophyte Ochromonas danica under photoautotrophic and mixotrophic growth. World J Microbiol Biotechnol 30(12):3111–3119CrossRefGoogle Scholar
  2. Biermann U, Bornscheuer U, Meier MAR, Metzger JO, Schäfer HJ (2011) Oils and fats as renewable raw materials in chemistry. Angew Chem Int Ed 50(17):3854–3871.  https://doi.org/10.1002/anie.201002767 CrossRefGoogle Scholar
  3. Borowitzka MA (1997) Microalgae for aquaculture: opportunities and constraints. J Appl Phycol 9(5):393–401CrossRefGoogle Scholar
  4. Canakci M, Sanli H (2008) Biodiesel production from various feedstocks and their effects on the fuel properties. J Ind Microbiol Biotechnol 35(5):431–441CrossRefGoogle Scholar
  5. Chi Z, Pyle D, Wen Z, Frear C, Chen S (2007) A laboratory study of producing docosahexaenoic acid from biodiesel-waste glycerol by microalgal fermentation. Process Biochem 42(11):1537–1545.  https://doi.org/10.1016/j.procbio.2007.08.008 CrossRefGoogle Scholar
  6. Dunstan GA, Volkman JK, Barrett SM, Garland CD (1993) Changes in the lipid composition and maximisation of the polyunsaturated fatty acid content of three microalgae grown in mass culture. J Appl Phycol 5(1):71–83.  https://doi.org/10.1007/bf02182424 CrossRefGoogle Scholar
  7. Fidalgo J, Cid A, Torres E, Sukenik A, Herrero C (1998) Effects of nitrogen source and growth phase on proximate biochemical composition, lipid classes and fatty acid profile of the marine microalga Isochrysis galbana. Aquaculture 166(1–2):105–116CrossRefGoogle Scholar
  8. Forsyth S (2015) Arachidonic acid and infant formulas. Pediatr Res 77(5):719–720CrossRefGoogle Scholar
  9. Gunstone FD (2004) The chemistry of oils and fats. Sources, Composition, Properties and Uses. Blackwell Publishing Ltd, Great Britain 345pGoogle Scholar
  10. Haines T, Aaronson S, Gellerman J, Schlenk H (1962) Occurrence of arachidonic and related acids in the protozoon Ochromonas danica. Nature 194(4835):1282–1283CrossRefGoogle Scholar
  11. Harel M, Koven W, Lein I, Bar Y, Behrens P, Stubblefield J, Zohar Y, Place AR (2002) Advanced DHA, EPA and ArA enrichment materials for marine aquaculture using single cell heterotrophs. Aquaculture 213(1–4):347–362CrossRefGoogle Scholar
  12. Harwood JL, Guschina IA (2009) The versatility of algae and their lipid metabolism. Biochimie 91(6):679–684CrossRefGoogle Scholar
  13. Hosseini M, Ju L-K (2015) Use of phagotrophic microalga Ochromonas danica to pretreat waste cooking oil for biodiesel production. J Am Oil Chem Soc 92(1):29–35CrossRefGoogle Scholar
  14. Jiang Y, Chen F (2000) Effects of medium glucose concentration and pH on docosahexaenoic acid content of heterotrophic Crypthecodinium cohnii. Process Biochem 35(10):1205–1209.  https://doi.org/10.1016/S0032-9592(00)00163-1 CrossRefGoogle Scholar
  15. Jiang Y, Chen F, Liang S-Z (1999) Production potential of docosahexaenoic acid by the heterotrophic marine dinoflagellate Crypthecodinium cohnii. Process Biochem 34(6):633–637.  https://doi.org/10.1016/S0032-9592(98)00134-4 CrossRefGoogle Scholar
  16. Krienitz L, Wirth M (2006) The high content of polyunsaturated fatty acids in Nannochloropsis limnetica (Eustigmatophyceae) and its implication for food web interactions, freshwater aquaculture and biotechnology. Limnologica 36(3):204–210.  https://doi.org/10.1016/j.limno.2006.05.002 CrossRefGoogle Scholar
  17. Li C, Ju L-K (2014) Conversion of wastewater organics into biodiesel feedstock through the predator-prey interactions between phagotrophic microalgae and bacteria. RSC Adv 4(83):44026–44029CrossRefGoogle Scholar
  18. Liang Y, Sarkany N, Cui Y (2009) Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions. Biotechnol Lett 31(7):1043–1049.  https://doi.org/10.1007/s10529-009-9975-7 CrossRefGoogle Scholar
  19. Lin Z, Raya A, Ju L-K (2014) Microalga Ochromonas danica fermentation and lipid production from waste organics such as ketchup. Process Biochem 49(9):1383–1392CrossRefGoogle Scholar
  20. Liu J, Huang J, Sun Z, Zhong Y, Jiang Y, Chen F (2011) Differential lipid and fatty acid profiles of photoautotrophic and heterotrophic Chlorella zofingiensis: assessment of algal oils for biodiesel production. Bioresour Technol 102(1):106–110.  https://doi.org/10.1016/j.biortech.2010.06.017 CrossRefGoogle Scholar
  21. Long JH, Aziz TN, Francis L, Ducoste JJ (2012) Anaerobic co-digestion of fat, oil, and grease (FOG): a review of gas production and process limitations. Process Saf Environ Prot 90(3):231–245CrossRefGoogle Scholar
  22. Metzger JO, Bornscheuer U (2006) Lipids as renewable resources: current state of chemical and biotechnological conversion and diversification. Appl Microbiol Biotechnol 71(1):13–22.  https://doi.org/10.1007/s00253-006-0335-4 CrossRefGoogle Scholar
  23. Montefrio MJ, Xinwen T, Obbard JP (2010) Recovery and pre-treatment of fats, oil and grease from grease interceptors for biodiesel production. Appl Energy 87(10):3155–3161CrossRefGoogle Scholar
  24. Moon M, Kim CW, Park W-K, Yoo G, Choi Y-E, Yang J-W (2013) Mixotrophic growth with acetate or volatile fatty acids maximizes growth and lipid production in Chlamydomonas reinhardtii. Algal Res 2(4):352–357.  https://doi.org/10.1016/j.algal.2013.09.003 CrossRefGoogle Scholar
  25. Ngo HL, Zafiropoulos NA, Foglia TA, Samulski ET, Lin W (2007) Efficient two-step synthesis of biodiesel from greases. Energy Fuel 22(1):626–634CrossRefGoogle Scholar
  26. Pastore C, Pagano M, Lopez A, Mininni G, Mascolo G (2015) Fat, oil and grease waste from municipal wastewater: characterization, activation and sustainable conversion into biofuel. Water Sci Technol 71(8):1151–1157CrossRefGoogle Scholar
  27. Pollero R, Brenner R, Dumm CG (1975) Comparative biosynthesis of polyethylenic fatty acids in Acanthamoeba castellanii and Ochromonas danica. Acta Physiol Lat Am 25(5):412–424Google Scholar
  28. Pringsheim E (1952) On the nutrition of Ochromonas. J Cell Sci 3(21):71–96Google Scholar
  29. Qiao H, Cong C, Sun C, Li B, Wang J, Zhang L (2016) Effect of culture conditions on growth, fatty acid composition and DHA/EPA ratio of Phaeodactylum tricornutum. Aquaculture 452:311–317CrossRefGoogle Scholar
  30. Shen Y, Linville JL, Urgun-Demirtas M, Mintz MM, Snyder SW (2015) An overview of biogas production and utilization at full-scale wastewater treatment plants (WWTPs) in the United States: challenges and opportunities towards energy-neutral WWTPs. Renew Sust Energ Rev 50:346–362CrossRefGoogle Scholar
  31. Springer M, Franke H, Pulz O (1994) Increase of the content of polyunsaturated fatty acids in Porphyridium cruentum by low-temperature stress and acetate supply. J Plant Physiol 143(4):534–537.  https://doi.org/10.1016/S0176-1617(11)81819-5 CrossRefGoogle Scholar
  32. Tan CK, Johns MR (1991) Fatty acid production by heterotrophic Chlorella saccharophila. Hydrobiologia 215(1):13–19.  https://doi.org/10.1007/bf00005896 CrossRefGoogle Scholar
  33. Tanaka T, Yabuuchi T, Maeda Y, Nojima D, Matsumoto M, Yoshino T (2017) Production of eicosapentaenoic acid by high cell density cultivation of the marine oleaginous diatom Fistulifera solaris. Bioresour Technol 245:567–572.  https://doi.org/10.1016/j.biortech.2017.09.005 CrossRefGoogle Scholar
  34. Van Wychen S, Laurens L (2013) Determination of total lipids as fatty acid methyl esters (FAME) by in situ transesterification: laboratory analytical procedure (LAP). National Renewable Energy Laboratory (NREL), GoldenGoogle Scholar
  35. Vogel G, Eichenberger W (1992) Betaine lipids in lower plants. Biosynthesis of DGTS and DGTA in Ochromonas danica (Chrysophyceae) and the possible role of DGTS in lipid metabolism. Plant Cell Physiol 33(4):427–436Google Scholar
  36. Wallace T, Gibbons D, O'Dwyer M, Curran TP (2017) International evolution of fat, oil and grease (FOG) waste management–a review. J Environ Manag 187:424–435CrossRefGoogle Scholar
  37. Wang L, Li Y, Chen P, Min M, Chen Y, Zhu J, Ruan RR (2010) Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp. Bioresour Technol 101(8):2623–2628.  https://doi.org/10.1016/j.biortech.2009.10.062 CrossRefGoogle Scholar
  38. Wang L, Aziz TN, Francis L (2013) Determining the limits of anaerobic co-digestion of thickened waste activated sludge with grease interceptor waste. Water Res 47(11):3835–3844CrossRefGoogle Scholar
  39. Wen Z-Y, Chen F (2001) Optimization of nitrogen sources for heterotrophic production of eicosapentaenoic acid by the diatom Nitzschia laevis. Enzym Microb Technol 29(6):341–347.  https://doi.org/10.1016/S0141-0229(01)00385-4 CrossRefGoogle Scholar
  40. Wilken S, Schuurmans JM, Matthijs HC (2014) Do mixotrophs grow as photoheterotrophs? Photophysiological acclimation of the chrysophyte Ochromonas danica after feeding. New Phytol 204(4):882–889CrossRefGoogle Scholar
  41. Xiao S, Ju L-K (2018) Energy-efficient ultrasonic release of bacteria and particulates to facilitate ingestion by phagotrophic algae for waste sludge treatment and algal biomass and lipid production. Chemosphere 209:588–598.  https://doi.org/10.1016/j.chemosphere.2018.06.120 CrossRefGoogle Scholar
  42. Yongmanitchai W, Ward OP (1991) Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions. Appl Environ Microbiol 57(2):419–425Google Scholar
  43. Zhu L, Wang Z, Shu Q, Takala J, Hiltunen E, Feng P, Yuan Z (2013) Nutrient removal and biodiesel production by integration of freshwater algae cultivation with piggery wastewater treatment. Water Res 47(13):4294–4302CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemical and Biomolecular EngineeringThe University of AkronAkronUSA

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