Applied Microbiology and Biotechnology

, Volume 103, Issue 1, pp 113–123 | Cite as

Engineering of the baculovirus expression system for optimized protein production

  • María Martínez-Solís
  • Salvador HerreroEmail author
  • Alexandra M. Targovnik


Baculoviruses are arthropod-specific large circular double-stranded DNA viruses successfully used for the control of multiple insect pests. In addition to their application in pest control, baculoviruses have become a versatile and powerful eukaryotic vector for the production of large quantities of recombinant proteins for research and biomedical purposes. Since the first recombinant protein was expressed in 1983 using the baculovirus expression system (BEVS), different strategies have been developed for the generation of recombinant viruses and to increase the stability, yield, and posttranslational modifications of recombinant proteins. In this review, we summarize the main methods and elements playing a role in the BEVS emphasizing recent progresses and future developments with respect to the main aspects involved in protein production using the BEVS.


Nucleopolyhedrovirus Protein expression Insect cells AcMNPV Insect biofactory 



This study was partially supported by Generalitat Valenciana (GVPROMETEOII-2015-001), by the Spanish Ministry of Economy, Industry and Competitiveness-European FEDER funds (grant AGL2014-57752-C2-2R), and by ANPCyT-FONCyT (PICT 2015-1992). AMT is a member of the Research Career of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.


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© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Genetics and Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI-BIOTECMED)Universitat de ValènciaBurjassotSpain
  2. 2.Facultad de Farmacia y Bioquímica.Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de BiotecnologíaUniversidad de Buenos AiresBuenos AiresArgentina
  3. 3.Instituto de Nanobiotecnología (NANOBIOTEC)CONICET-Universidad de Buenos AiresBuenos AiresArgentina

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