Identification of novel bacteriophage vB_EcoP-EG1 with lytic activity against planktonic and biofilm forms of uropathogenic Escherichia coli
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Urinary tract infections are one of the most common infectious diseases worldwide. Uropathogenic Escherichia coli (UPEC) is a major cause of unary tract infection. Due to increasing prevalence of multidrug resistance, alternative methods to eradicate the UPECs are urgently needed. In this respect, phage therapy has been demonstrated to be a good candidate. Here, we described a novel bacteriophage named vB_EcoP-EG1, which can infect several strains of UPEC. Phage morphology and genome sequencing analysis show that vB_EcoP-EG1 belongs to the T7-like Podoviridae. vB_EcoP-EG1 possesses a genome (39,919 bp) containing 51 predicted genes and 149 bp terminal repeats. vB_EcoP-EG1 genome does not encode toxic proteins or proteins related to lysogeny. And no known virulent proteins were found in purified phage particles by mass spectrometry. vB_EcoP-EG1 appeared to be relatively specific and sensitive to clinical UPEC strains, which could infect 10 out of 21 clinical multidrug-resistant UPEC strains. In addition, vB_EcoP-EG1 suspension can eliminate biofilm formed by E. coli MG1655 and multidrug-resistant UPEC strain 390G7. Therefore, we concluded that vB_EcoP-EG1 has desirable characteristics for potential therapy, which may serve as an alternative to antibiotic therapy against urinary tract infections caused by multidrug-resistant UPEC.
KeywordsPhage Unary tract infection Genome sequencing Mass spectrometry UPEC
We would like to thank Dr. Xuesen Zhang (Nanjing Medical University) for providing assistance with the English grammar.
This work was supported by the National Natural Science Foundation of China (81501797) and the Natural Science Foundation of Jiangsu Province (BK20151558).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Human and animal rights and informed consent
Clinical strains were isolated from clinical samples of patients in the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
- Birgy A, Doit C, Mariani-Kurkdjian P, Genel N, Faye A, Arlet G, Bingen E (2011) Early detection of colonization by VIM-1-producing Klebsiella pneumoniae and NDM-1-producing Escherichia coli in two children returning to France. J Clin Microbiol 49:3085–3087. https://doi.org/10.1128/JCM.00540-11 CrossRefPubMedPubMedCentralGoogle Scholar
- Danis-Wlodarczyk K, Olszak T, Arabski M, Wasik S, Majkowska-Skrobek G, Augustyniak D, Gula G, Briers Y, Jang HB, Vandenheuvel D, Duda KA, Lavigne R, Drulis-Kawa Z (2015) Characterization of the newly isolated lytic bacteriophages KTN6 and KT28 and their efficacy against Pseudomonas aeruginosa biofilm. PLoS One 10:e0137015. https://doi.org/10.1371/journal.pone.0137015. CrossRefPubMedPubMedCentralGoogle Scholar
- Galtier M, De Sordi L, Maura D, Arachchi H, Volant S, Dillies MA, Debarbieux L (2016) Bacteriophages to reduce gut carriage of antibiotic resistant uropathogens with low impact on microbiota composition. Environ Microbiol 18:2237–2245. https://doi.org/10.1111/1462-2920.13284 CrossRefPubMedGoogle Scholar
- Guiton PS, Cusumano CK, Kline KA, Dodson KW, Han Z, Janetka JW, Henderson JP, Caparon MG, Hultgren SJ (2012) Combinatorial small-molecule therapy prevents uropathogenic Escherichia coli catheter-associated urinary tract infections in mice. Antimicrob Agents Chemother 56:4738–4745. https://doi.org/10.1128/AAC.00447-12 CrossRefPubMedPubMedCentralGoogle Scholar
- Guo F, Liu Z, Fang PA, Zhang Q, Wright ET, Wu W, Zhang C, Vago F, Ren Y, Jakana J, Chiu W, Serwer P, Jiang W (2014) Capsid expansion mechanism of bacteriophage T7 revealed by multistate atomic models derived from cryo-EM reconstructions. Proc Natl Acad Sci U S A 111:E4606–E4614. https://doi.org/10.1073/pnas.1407020111 CrossRefPubMedPubMedCentralGoogle Scholar
- Johnson JR, Tchesnokova V, Johnston B, Clabots C, Roberts PL, Billig M, Riddell K, Rogers P, Qin X, Butler-Wu S, Price LB, Aziz M, Nicolas-Chanoine MH, Debroy C, Robicsek A, Hansen G, Urban C, Platell J, Trott DJ, Zhanel G, Weissman SJ, Cookson BT, Fang FC, Limaye AP, Scholes D, Chattopadhyay S, Hooper DC, Sokurenko EV (2013) Abrupt emergence of a single dominant multidrug-resistant strain of Escherichia coli. J Infect Dis 207:919–928. https://doi.org/10.1093/infdis/jis933 CrossRefPubMedPubMedCentralGoogle Scholar
- Lo E, Nicolle LE, Coffin SE, Gould C, Maragakis LL, Meddings J, Pegues DA, Pettis AM, Saint S, Yokoe DS (2014) Strategies to prevent catheter-associated urinary tract infections in acute care hospitals: 2014 update. Infect Control Hosp Epidemiol 35:464–479. https://doi.org/10.1086/675718 CrossRefPubMedGoogle Scholar
- Nishikawa H, Yasuda M, Uchiyama J, Rashel M, Maeda Y, Takemura I, Sugihara S, Ujihara T, Shimizu Y, Shuin T, Matsuzaki S (2008) T-even-related bacteriophages as candidates for treatment of Escherichia coli urinary tract infections. Arch Virol 153:507–515. https://doi.org/10.1007/s00705-007-0031-4 CrossRefPubMedGoogle Scholar
- Niveditha S, Pramodhini S, Umadevi S, Kumar S, Stephen S (2012) The isolation and the biofilm formation of uropathogens in the patients with catheter associated urinary tract infections (UTIs). J Clin Diagn Res 6:1478–1482. https://doi.org/10.7860/JCDR/2012/4367.2537 CrossRefPubMedPubMedCentralGoogle Scholar
- Regeimbal JM, Jacobs AC, Corey BW, Henry MS, Thompson MG, Pavlicek RL, Quinones J, Hannah RM, Ghebremedhin M, Crane NJ, Zurawski DV, Teneza-Mora NC, Biswas B, Hall ER (2016) Personalized therapeutic cocktail of wild environmental phages rescues mice from Acinetobacter baumannii wound infections. Antimicrob Agents Chemother 60:5806–5816. https://doi.org/10.1128/AAC.02877-15 CrossRefPubMedPubMedCentralGoogle Scholar
- Synnott AJ, Kuang Y, Kurimoto M, Yamamichi K, Iwano H, Tanji Y (2009) Isolation from sewage influent and characterization of novel Staphylococcus aureus bacteriophages with wide host ranges and potent lytic capabilities. Appl Environ Microbiol 75:4483–4490. https://doi.org/10.1128/AEM.02641-08 CrossRefPubMedPubMedCentralGoogle Scholar
- Tabib-Salazar A, Liu B, Shadrin A, Burchell L, Wang Z, Wang Z, Goren MG, Yosef I, Qimron U, Severinov K, Matthews SJ, Wigneshweraraj S (2017) Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein. Nucleic Acids Res 45:7697–7707. https://doi.org/10.1093/nar/gkx370 CrossRefPubMedPubMedCentralGoogle Scholar
- Vandamme EJ, Mortelmans K (2018) A century of bacteriophage research and applications: impact on biotechnology, health, ecology and economy. J Chem Technol Biotechnol. https://doi.org/10.1002/jctb.5810
- Xu Y, Ma Y, Yao S, Jiang Z, Pei J, Cheng C (2016) Characterization, genome sequence, and analysis of Escherichia phage CICC 80001, a bacteriophage infecting an efficient L-aspartic acid producing Escherichia coli. Food Environ Virol 8:18–26. https://doi.org/10.1007/s12560-015-9218-0 CrossRefPubMedGoogle Scholar