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Applied Microbiology and Biotechnology

, Volume 103, Issue 1, pp 489–503 | Cite as

Enhancement of corn stover conversion to carboxylates by extrusion and biotic triggers in solid-state fermentation

  • Antonella Marone
  • Eric TrablyEmail author
  • Hélène Carrère
  • Pacôme Prompsy
  • Fabienne Guillon
  • Maud Joseph-Aimé
  • Abdellatif Barakat
  • Nour Fayoud
  • Nicolas Bernet
  • Renaud Escudié
Environmental biotechnology

Abstract

Solid-state fermentation is a potential technology for developing lignocellulosic biomass-based biorefineries. This work dealt with solid-state fermentation for carboxylates production from corn stover, as building blocks for a lignocellulosic feedstock-based biorefinery. The effect of extrusion pretreatment, together with the action of a microbial consortia and hydrolytic enzymes as biotic triggers, was investigated on corn stover conversion, microbial metabolic pathways, and populations. The extrusion caused changes in the physical and morphological characteristics, without altering the biochemical composition of the corn stover. Extrusion also led to remarkable differences in the composition of the indigenous microbial population of the substrate. Consequently, it affected the structure of community developed after fermentation and the substrate conversion yield, which increased by 118% (from 23 ± 4 gCOD/kgVSi obtained with raw substrate to 51 ± 1 gCOD/kgVSi with extruded corn stover) with regard to self-fermentation experiments. The use of activated sludge as inoculum further increased the total substrate conversion into carboxylates, up to 60 ± 2 gCOD/kgVSi, and shaped the microbial communities (mainly composed of bacteria from the Clostridia and Bacteroidia classes) with subsequent homogenization of the fermentation pathways. The addition of hydrolytic enzymes into the reactors further increased the corn stover conversion, leading to a maximum yield of 142 ± 1 gCOD/kgVSi. Thus, extrusion pretreatment combined with the use of an inoculum and enzyme addition increased by 506% corn stover conversion into carboxylates. Beside biomass pretreatment, the results of this study indicated that biotic factor greatly impacted solid-state fermentation by shaping the microbial communities and related metabolic pathways.

Keywords

Solid-state fermentation Mixed culture Pretreatment Lignocellulosic biomass Carboxylates Environmental biorefinery 

Notes

Acknowledgements

We thank Valérie Méchin for her help for the lignin content measurements. We are indebted to Marie-Jeanne Crepeau for her technical assistance. We thank Marie-Françoise Devaux for her help in image acquisition and analysis, and Xavier Falourd and the IBISA/BioGenOuest Biopolymers Structural Biology platform for the measurement of cellulose crystallinity index by solid-state 13C nuclear magnetic resonance. The authors gratefully acknowledge Gaëlle Santa-Catalina for molecular biology practices.

Funding

This study was funded by The French National Research Agency (ANR) project 14-CE19-0013, Hi-Solids.

Compliance with ethical standards

Conflict of interest

Antonella Marone declares that she has no conflict of interest. Eric Trably declares that he has no conflict of interest. Helene Carrere declares that she has no conflict of interest. Pacome Prompsy declares that he has no conflict of interest. Fabienne Guillon declares that she has no conflict of interest. Maud Joseph-Aimé declares that she has no conflict of interest. Abdellatif Barakat declares that he has no conflict of interest. Nour Fayoud declares that she has no conflict of interest. Nicolas Bernet declares that he has no conflict of interest. Renaud Escudié declares that he has no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2018_9463_MOESM1_ESM.pdf (323 kb)
ESM 1 (PDF 322 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Antonella Marone
    • 1
    • 2
  • Eric Trably
    • 1
    Email author return OK on get
  • Hélène Carrère
    • 1
  • Pacôme Prompsy
    • 1
  • Fabienne Guillon
    • 3
  • Maud Joseph-Aimé
    • 3
  • Abdellatif Barakat
    • 4
  • Nour Fayoud
    • 4
    • 5
  • Nicolas Bernet
    • 1
  • Renaud Escudié
    • 1
  1. 1.LBE, INRAUniv MontpellierNarbonneFrance
  2. 2.GENOCOV, Departament d’Enginyeria Química, Biològica i Ambiental, Escola d’EnginyeriaUniversitat Autònoma de BarcelonaBarcelonaSpain
  3. 3.UR1268 BIA, INRANantesFrance
  4. 4.UMR, IATE, CIRAD, Montpellier SupAgro, INRAUniversité de MontpellierMontpellierFrance
  5. 5.Mohammed VI Polytechnic UniversityBen GuerirMorocco

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