Polyhydroxyalkanoate synthesis based on glycerol and implementation of the process under conditions of pilot production
- 172 Downloads
The present study addresses the synthesis and properties of polyhydroxyalkanoates (PHA) of different composition synthesized by Cupriavidus eutrophus B-10646 using glycerol as a carbon substrate. Poly(3-hydroxybutyrate) [P(3HB)] was effectively synthesized in fed-batch culture in a 30-L fermenter on glycerol of various purification degrees, with 99.5, 99.7, and 82.1% content of the main component. Purified glycerol (99.7%) was used for 150-L pilot scale fermentation. The total biomass and P(3HB) concentration reached 110 and 85.8 g/L, respectively, after 45 h of fed-batch fermentation. An average volumetric productivity of P(3HB) was 1.83 g/(L h). The degree of crystallinity and molecular weight of P(3HB) synthesized on glycerol were lower than and temperature characteristics were the same as those of P(3HB) synthesized on sugars.
KeywordsGlycerol Polyhydroxyalkanoates Synthesis Productivity Properties
This study was financially supported by the project “Agropreparations of the new generation: a strategy of construction and realization” (Agreement No. 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, “On measures designed to attract leading scientists to the Russian institutions of higher learning”.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals, performed by any of the authors.
- Campos MI, Figueiredo TVB, Sousa LS, Druzian JI (2014) The influence of crude glycerin and nitrogen concentrations on the production of PHA by Cupriavidus necator using a response surface methodology and its characterizations. Ind Crop Prod 52:338–346. https://doi.org/10.1016/j.indcrop.2013.11.008 CrossRefGoogle Scholar
- Cavalheiro JMBT, Raposo RSM, de Almeida MCMD, Cesário MT, Sevrin C, Grandfils C, da Fonseca MMR (2012) Effect of cultivation parameters on the production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate) by Cupriavidus necator using waste glycerol. Bioresour Technol 111:391–397. https://doi.org/10.1016/j.biortech.2012.01.176 CrossRefPubMedGoogle Scholar
- Fernández-Dacosta C, Posada JA, Kleerebezem R, Cuellar MC, Ramirez A (2015) Microbial community-based polyhydroxyalkanoates (PHAs) production from wastewater: techno-economic analysis and ex-ante environmental assessment. Bioresour Technol 185:368–377. https://doi.org/10.1016/j.biortech.2015.03.025 CrossRefPubMedGoogle Scholar
- García IL, López JA, Dorado MP, Kopsahelis N, Alexandri M, Papanikolaou S, Villar MA, Koutinas AA (2013) Evaluation of by-products from the biodiesel industry as fermentation feedstock for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production by Cupriavidus necator. Bioresour Technol 130:16–22. https://doi.org/10.1016/j.biortech.2012.11.088 CrossRefPubMedGoogle Scholar
- Global Renewable Fuels Alliance. World ethanol production playing an increasing role in energy security. http://www.globalrfa.org/pr_021111.php. Accessed 04 Dec 2012
- Hermann-Krauss C, Koller M, Muhr A, Fasl H, Stelzer F, Braunegg G (2013) Archaeal production of polyhydroxyalkanoates (PHA) co- and terpolyesters from biodiesel industry-derived by-products. Archaea Article ID 129268, 10 pages. https://doi.org/10.1155/2013/129268
- Laycock B, Peter H, Pratt S, Werker A, Lant P (2013) The chemomechanical properties of microbial polyhydroxyalkanoate. Prog Polym Sci 38:536–583. https://doi.org/10.1016/j.progpolymsci.2012.06.003 CrossRefGoogle Scholar
- Nakamura M, Iso H, Kitamura A, Imano H, Noda H, Kiyama M, Sato S, Yamagishi K, Nishimura K, Nakai M, Vesper HW, Teramoto T, Miyamoto Y (2016) Comparison between the triglycerides standardization of routine methods used in Japan and the chromotropic acid reference measurement procedure used by the CDC lipid standardization Programme. Ann Clinic Biochem 53:632–639. https://doi.org/10.1177/0004563215624461 CrossRefGoogle Scholar
- Papanikolaou S, Fakas S, Fick M, Chevalot I, Galiotou-Panayotou M, Komaitis M, Marc I, Aggelis G (2007) Biotechnological valorization of raw glycerol discharged after bio-diesel (fatty acid methyl esters) manufacturing process: production of 1,3-propanediol, citric acid and single cell oil. Biomass Bioenergy 32:60–71. https://doi.org/10.1016/j.biombioe.2007.06.007 CrossRefGoogle Scholar
- Rodríguez-Contreras A, Koller M, de Sousa Dias MM, Calafell-Monfort M, Braunegg G, Marqués-Calvo MS (2015) Influence of glycerol on poly(3-hydroxybutyrate) production by Cupriavidus necator and Burkholderias acchari. Biochem Eng J 94:50–57. https://doi.org/10.1016/j.bej.2014.11.007 CrossRefGoogle Scholar
- Standard methods for the examination of water and wastewater (1989) American Publication of Health Association, WashingtonGoogle Scholar
- Statista (2018) Leading biodiesel producers worldwide in 2017, by country (in billion liters). https://www.statista.com/statistics/271472/biodiesel-production-in-selected-countries
- Taidi B, Anderson A, Dawes EA, Byrom D (1994) Effect of carbon sources and concentration on the molecular mass of poly(3-hydroxybutyrate) produced by Methylobacterium extorquens and Alcaligenes eutrophus. Appl Microbiol Biotechnol 40:786–790. https://doi.org/10.1007/BF00173975 CrossRefGoogle Scholar
- Tsuge T, Ko T, Tago M, Abe H (2013) Effect of glycerol and its analogs on polyhydroxyalkanoate biosynthesis by recombinant Ralstonia eutropha: a quantitative structure-activity relationship study of chain transfer agents. Polym Degrad Stab 98:1586–1590. https://doi.org/10.1016/j.polymdegradstab.2013.06.026 CrossRefGoogle Scholar
- Volova TG, Kalacheva GS (1990) Polyhydroxybutyrate – thermoplastic biodegradable plymer (obtaining, properties, application). Preprint 131B, Krasnoyarsk. 47 p (in Russian)Google Scholar
- Volova TG, Shishatskaya EI, Sinskey AJ (2013a) Degradable polymers: Production, properties, applications. Nova Science Pub. Inc., New YorkGoogle Scholar
- Volova T, Kiselev E, Shishatskaya E, Zhila N, Boyandin A, Syrvacheva D, Vinogradova O, Kalacheva G, Vasiliev A, Peterson I (2013b) Cell growth and PHA accumulation from CO2 and H2 of a hydrogen-oxidizing bacterium, Cupriavidus eutrophus В-10646. Bioresour Technol 146:215–222. https://doi.org/10.1016/j.biortech.2013.07.070 CrossRefPubMedGoogle Scholar
- Volova TG, Kiselev EG, Vinogradova ON, Nikolaeva ED, Chistyakov AA, Sukovatyi AG, Shishatskaya EI (2014) A glucose-utilizing strain, Сupriavidus eutrophus В-10646: growth kinetics, characterization and synthesis of multicomponent PHAs. PLoS One 9:e87551. https://doi.org/10.1371/journal.pone.0087551 CrossRefPubMedPubMedCentralGoogle Scholar