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Applied Microbiology and Biotechnology

, Volume 103, Issue 1, pp 361–374 | Cite as

Multi-omics metabolism analysis on irradiation-induced oxidative stress to Rhodotorula glutinis

  • Guiping Gong
  • Luo Liu
  • Xu ZhangEmail author
  • Tianwei Tan
Applied microbial and cell physiology
  • 137 Downloads

Abstract

Oxidative stress is induced in many organisms by various natural abiotic factors including irradiation. It has been demonstrated that it significantly improves growth rate and lipid production of Rhodotorula glutinis. However, the specific mechanism of how irradiation influences the metabolism of R. glutinis remains still unavailable. To investigate and better understand the mechanisms involved in irradiation-induced stress resistance in R. glutinis, a multi-omics metabolism analysis was implemented. The results confirmed that irradiation indeed not only improved cell biomass but also accelerated the production of carotenoids and lipids, especially neutral lipid. Compared with the control, metabolome profiling in the group exposed to irradiation exhibited an obvious difference in the activation of the tricarboxylic acid cycle and triglyceride (TAG) production. The results of proteome analysis (data are available via ProteomeXchange with identifier PXD009678) showed that 423 proteins were changed significantly, and proteins associated with protein folding and transport, the Hsp40 and Sec12, were obviously upregulated, indicating that cells responded to irradiation by accelerating the protein folding and transport of correctly folded proteins as well as enhanced the degradation of misfolded proteins. A significant upregulation of the carotenoid biosynthetic pathway was observed which revealed that increased carotenoid content is a cellular defense mechanism against oxidative stress generated by irradiation. Therefore, the results of comprehensive omics analysis provide intensive insights on the response mechanism of R. glutinis to irradiation-induced oxidative stress which could be helpful for using irradiation as an effective strategy to enhance the joint production of the neutral lipid and carotene.

Keywords

Rhodotorula glutinis Irradiation Stress response Multi-omics analysis 

Notes

Funding

This study was funded by the National Key Research and Development Program of China (grant number 2017YFB0306803) and the 111 project (grant number B13005).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2018_9448_MOESM1_ESM.pdf (1018 kb)
ESM 1 (PDF 1017 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and TechnologyBeijing University of Chemical TechnologyBeijingPeople’s Republic of China

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