Applied Microbiology and Biotechnology

, Volume 102, Issue 21, pp 9389–9398 | Cite as

Microbial nitrogen removal of ammonia wastewater in poly (butylenes succinate)-based constructed wetland: effect of dissolved oxygen

  • Huaqing Liu
  • Zhen Hu
  • Yijin Zhang
  • Jian ZhangEmail author
  • Huijun Xie
  • Shuang Liang
Environmental biotechnology


Constructed wetland (CW) is popular in wastewater treatment for its prominent advantage of low construction and operation cost. However, the nitrogen removal in conventional CW is usually limited by the low dissolved oxygen (DO) and insufficient electron donor. This paper investigated the nitrogen removal performance and mechanisms in the poly (butylenes succinate)-based CW (PBS-CW) while treating ammonia wastewater under different DO levels. The average DO contents in limited-aeration and full-aeration phases were 1.68 mg L−1 and 5.71 mg L−1, respectively. Results indicated that, with the ammonia nitrogen loading rate of 25 g N m−3 day−1, total nitrogen removal ratios in the PBS-CW under the limited-aeration and full-aeration phases were 72% and 99%, respectively. Combined analyses revealed that simultaneous nitrification and denitrification (SND) via nitrite/nitrate were the main microbial nitrogen removal pathways in the aeration phase of the PBS-CW (> 89%). The microbial carrier of biodegradable material was believed to play a significant role in prompting SND performance while dealing with low C/N wastewater. Due to the coexistence of micro-anaerobic zone and carbon supply inside the coated biofilm, the high DO level in the PBS-CW increased the abundance of the nitrifying bacteria (amoA and nxrA), denitrifying bacteria (narG, nirK, nirS, and nosZ), and even anammox bacteria (anammox 16s rRNA). These features are beneficial to many microbial processes which require the simultaneous aerobic, anoxic, and anaerobic environment.


Constructed wetland Ammonia nitrogen Poly (butylenes succinate) Dissolved oxygen Microbial nitrogen removal 


Funding information

This study was supported by the China Major Science and Technology Program for Water Pollution Control and Treatment (No.2017ZX07101003), National Natural Science Foundation of China (No. 51720105013), Shandong Provincial Key Research and Development Plan (No. 2017GSF216011), and Fundamental Research Funds of Shandong University (No.2017JC025).

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

253_2018_9386_MOESM1_ESM.pdf (605 kb)
ESM 1 (PDF 604 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Huaqing Liu
    • 1
  • Zhen Hu
    • 1
  • Yijin Zhang
    • 2
  • Jian Zhang
    • 1
    • 3
    Email author
  • Huijun Xie
    • 4
  • Shuang Liang
    • 1
  1. 1.Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science & EngineeringShandong UniversityJinanChina
  2. 2.Environmental Engineering Co., Ltd.Shandong Academy of Environmental ScienceJinanChina
  3. 3.State key Laboratory of Microbial TechnologyShandong UniversityJinanPeople’s Republic of China
  4. 4.Environment Research InstituteShandong UniversityJinanChina

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