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Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes

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Abstract

Proteins were obtained from effluent of a starch manufacture by using different isolation temperatures (40, 60, 80, and 100 °C). The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C. The protein samples of 80 °C exhibited the highest antifungal activity and its average value of IC50 against five strains of two Fusarium species was determined in average at 0.18 mg ml−1. The 80 °C protein samples consisted predominantly of low-molecular proteins (7–17 kDa) identified as potato tuber protease inhibitors I and II. Predominantly, protease inhibitors II were identified for the protein samples obtained by 100 °C and here we identified 7 spots in comparison with 12 identified for the 80 °C samples. Samples of 40 and 60 °C with low antifungal activities represent high variability of detected and identified proteins. We identified various representatives of aspartic, cysteine, and serine protease inhibitors in both types of samples. These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors. Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures. At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.

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Funding

The work was supported by the Czech Science Foundation, project nos. 522/09/1693, and by the Grant Agency of the University of South Bohemia in České Budějovice, project GAJU 112/2016/Z. The financial support of the MS measurements at the Proteomics Core Facility was received from the CIISB research infrastructure project LM2015043 that was funded by MEYS CR.

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Correspondence to Veronika Bártová.

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This article does not contain any studies with human participants or animals by any of the authors.

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The author declares that there is no competing interests.

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Bártová, V., Bárta, J., Vlačihová, A. et al. Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes. Appl Microbiol Biotechnol 102, 10551–10560 (2018). https://doi.org/10.1007/s00253-018-9373-y

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Keywords

  • Potato
  • Solanum tuberosum L.
  • Thermally stable proteins
  • Protease inhibitors
  • Antifungal activity