Adhesion of Rhodococcus ruber IEGM 342 to polystyrene studied using contact and non-contact temperature measurement techniques
- 36 Downloads
Adhesion of industrially important bacteria to solid carriers through the example of actinobacterium Rhodococcus ruber IEGM 342 adhered to polystyrene was studied using real-time methods, such as infrared (IR) thermography and thermometry with platinum resistance (PR) detectors. Dynamics of heat rate and heat production was determined at early (within first 80 min) stages of rhodococcal cell adhesion. Heat rate was maximal (1.8 × 10−3–2.7 × 10−3 W) at the moment of cell loading. Heat production was detected for the entire length of adhesion, and its dynamics depended on concentration of rhodococcal cells. At high (1 × 1010 CFU/ml) cell concentration, a stimulative (in 1.7 and 1.4 times consequently) effect of polystyrene treatment with Rhodococcus-biosurfactant on the number of adhered rhodococcal cells and cumulative heat production at rhodococcal cell adhesion was revealed. The values of heat flows (heat rate 0.3 × 10−3–2.7 × 10−3 W, heat production up to 8.2 × 10−3 J, and cumulative heat production 0.20–0.53 J) were 5–30 times higher than those published elsewhere that indicated high adhesive activity of R. ruber IEGM 342 towards polystyrene. To analyze experimental results and predict effects of boundary conditions on the temperature distribution, a mathematical model for heating a polystyrene microplate with distributed heat sources has been developed. Two independent experimental methods and the numerical modeling make it possible to verify the experimental results and to propose both contact and non-contact techniques for analyzing kinetics of bacterial adhesion.
KeywordsBacterial adhesion Adhesion thermodynamics Infrared thermography Platinum resistance thermometers Rhodococcus actinobacteria
This work was performed as part of the State Tasks 6.3330.2017/4.6, 116012010212, and the State Registration Theme No. 01201353247 from the RF Ministry of Science and Higher Education.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Andrews CS, Denyer SP, Hall B, Hanlon GW, Lloyd AW (2001) A comparison of the use of an ATP-based bioluminescent assay and image analysis for the assessment of bacterial adhesion to standard HEMA and biomimetic soft contact lenses. Biomaterials 22:3225–3233. https://doi.org/10.1016/S0142-9612(01)00160-0 CrossRefPubMedGoogle Scholar
- Astasov-Frauenhoffer M, Braissant O, Hauser-Gerspach I, Daniels AU, Wirz D, Weiger R, Waltimo T (2011) Quantification of vital adherent Streptococcus sanguinis cells on protein-coated titanium after disinfectant treatment. J Mater Sci Mater Med 22:2045–2051. https://doi.org/10.1007/s10856-011-4377-5 CrossRefPubMedGoogle Scholar
- Bouderbala K, Nouira H, Girault M, Videcoq E (2016) Experimental thermal regulation of an ultra-high precision metrology system by combining modal identification method and model predictive control. Appl Therm Eng 104:504–515. https://doi.org/10.1016/j.applthermaleng.2016.05.085 CrossRefGoogle Scholar
- Chizzotti ML, Heiderich D, Aziani WLB, Ladeira MM, Valente EEL, Yanagi Junior T, Chizzotti FHM, Schiassi L, Lourençoni D (2013) Protein turnover and infrared thermography in Nellore bulls classified for residual feed intake. In: Oltjen JW (ed) Energy and protein metabolism and nutrition is sustainable animal protection. Wageningen Academic Publishers, Wageningen, pp 125–126CrossRefGoogle Scholar
- Diao M, Taran E, Mahler S, Nguyen TAH, Nguyen AV (2014) Quantifying adhesion of acidophilic bioleaching bacteria to silica and pyrite by atomic force microscopy with a bacterial probe. Colloids Surf B: Biointerfaces 115:229–236. https://doi.org/10.1016/j.colsurfb.2013.11.047 CrossRefPubMedGoogle Scholar
- Dinamarca MA, Orellana L, Aguirre J, Baeza P, Espinoza G, Canales C, Ojeda J (2014) Biodesulfurization of dibenzothiophene and gas oil using a bioreactor containing a catalytic bed with Rhodococcus rhodochrous immobilized on silica. Biotechnol Lett 36:1649–1652. https://doi.org/10.1007/s10529-014-1529-y CrossRefGoogle Scholar
- Gallardo-Moreno AM, González-Martín ML, Pérez-Giraldo C, Garduño E, Bruque JM, Gómez-García AC (2002) Thermodynamic analysis of growth temperature dependence in the adhesion of Candida parapsilosis to polystyrene. Appl Environ Microbiol 68:2610–2613. https://doi.org/10.1128/AEM.68.5.2610 CrossRefPubMedPubMedCentralGoogle Scholar
- Ivshina IB, Kuyukina MS, Krivoruchko AV, Plekhov OA, Naimark OB, Podorozhko EA, Lozinsky VI (2013) Biosurfactant-enhanced immobilization of hydrocarbon-oxidizing Rhodococcus ruber on sawdust. Appl Microbiol Biotechnol 97:5315–5327. https://doi.org/10.1007/s00253-013-4869-y CrossRefPubMedGoogle Scholar
- Kuyukina MS, Ivshina IB, Osipenko MA, Nyashin YI, Tyulenyova AN, Serebrennikova MK, Krivoruchko AV (2007) A kinetic model of bacterial cell immobilization process on the solid carrier. Russ J Biomech 11:76–84Google Scholar
- Kuyukina MS, Ivshina IB, Serebrennikova MK, Krivoruchko AV, Korshunova IO, Peshkur TA, Cunningham CJ (2017) Oilfield wastewater biotreatment in a fluidized-bed bioreactor using co-immobilized Rhodococcus cultures. J Environ Chem Eng 5:1252–1260. https://doi.org/10.1016/j.jece.2017.01.043 CrossRefGoogle Scholar
- Ng EYK (2009) A review of thermography as promising non-invasive detection modality for breast tumor. Int J Therm Sci 48:849–859. https://doi.org/10.1016/j.ijthermalsci.2008.06.015 CrossRefGoogle Scholar
- Omarova EO, Lobakova ES, Dolnikova GA, Nekrasova VV, Idiatulov RK, Kashcheeva PB, Perevertailo NG, Dedov AG (2012) Immobilization of bacteria on polymer matrices for degradation of crude oil and oil products. Mosc Univ Biol Sci Bull 67:24–30. https://doi.org/10.3103/S0096392512010063 CrossRefGoogle Scholar
- Vollmer M, Möllmann K-P (2010) Microsystems. In: Infrared thermal imaging. WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, pp 445–475Google Scholar