Roxithromycin regulates intestinal microbiota and alters colonic epithelial gene expression
The specialty of gastroenterology will be affected profoundly by the ability to modify the gastrointestinal microbiota through the use of antibiotics. This study investigated the in vivo effect of roxithromycin on gut bacteria and gene expression of colonic epithelial cells (CECs) using microbial 16S rDNA and colonic epithelial cell RNA sequencing, respectively. The results showed that roxithromycin distinctly lowered the microbial diversity in both the small intestine and cecum and altered the compositions of bacteria at both the phylum and genus levels, including the reduction of some bacteria beneficial to the hosts’ health. Eight decreased and 8 increased genera in the small intestine and 17 decreased and 4 increased genera of bacteria in the cecum were most affected by roxithromycin consumption. This consumption further altered the CECs’ expression of multiple genes. Thirty-one genes, which were significantly enriched in seven KEGG pathways and related to immune response, wound healing, and fibrosis, were significantly affected. Roxithromycin ingestion in healthy hosts, therefore, might lead to some undesirable consequences via affecting hosts’ gut microbiota and CECs. Our work offers a more comprehensive understanding of the impact of consuming roxithromycin on human health.
KeywordsRoxithromycin Gut microbiota Colonic epithelial cells Gene expression profile
We thank Ruixia Qiu and Bing Yu from the Department of Food Science and Engineering, Jinan University, for their contributions to the experiments of this study.
The program was funded by the National Natural Science Funds (No. 31471589) and the Fundamental Research Funds for the Central Universities (No. 21615404).
Compliance with ethical standard
Conflict of interest
All authors declare that they have no conflict of interest.
All Institutional Animal Care and Use Committee of Jinan University guidelines for the care and use of animals were followed.
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