Stability and oscillatory behavior of microbial consortium in continuous conversion of crude glycerol to 1,3-propanediol
- 141 Downloads
Microbial consortium is an alternative for bioconversion of crude glycerol to value-added products whereas concerns about the process stability in long-term operation existed. The aim of this study is to evaluate the feasibility of using an anaerobic microbial consortium as inoculum for continuous conversion of crude glycerol to 1,3-propanediol (1,3-PDO). Performances of continuous fermentations with the consortium inoculum were evaluated under different dilution rates and glycerol feed concentrations. The highest 1,3-PDO production of 57.86 g/L was achieved with a productivity of 5.55 g/(L·h). Analyses of kinetic data showed that the consortium maintained a consistent pattern for 1,3-PDO production under different operating conditions despite changes in community composition. The continuous fermentation by the consortium was able to operate for a longer period of time (31 volume changes) than that using pure culture (24 volume changes) with the average 1,3-PDO concentration of 53.52 g/L and productivity of 6.69 g/(L·h) under glycerol-excess condition, which could be contributed to the intraspecies diversity among Clostridium butyricum in the consortium. Under glycerol-limited conditions, however, a spontaneous oscillation of the consortium was observed after continuous operation for about 120 h, along with severe fluctuations of the microbial community. The oscillatory behavior could be reduced by increasing the dilution rates and was likely the metabolic feature of C. butyricum.
Keywords1,3-Propanediol Microbial consortium Continuous fermentation Clostridium butyricum Stability Oscillation
This work was supported by the National Natural Science Foundation of China (Grant No. 21476042) and the Fundamental Research Funds for the Central Universities (Grant No. DUT17ZD209).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Abbad-Andaloussi S, Guedon E, Spiesser E, Petitdemange H (1996) Glycerol dehydratase activity: the limiting step for 1,3-propanediol production by Clostridium butyricum DSM 5431. Lett Appl Microbiol 22(4):311–314. https://doi.org/10.1111/j.1472-765X.1996.tb01168.x CrossRefGoogle Scholar
- Chatzifragkou A, Papanikolaou S, Dietz D, Doulgeraki AI, Nychas GJ, Zeng AP (2011) Production of 1,3-propanediol by Clostridium butyricum growing on biodiesel-derived crude glycerol through a non-sterilized fermentation process. Appl Microbiol Biotechnol 91(1):101–112. https://doi.org/10.1007/s00253-011-3247-x CrossRefPubMedGoogle Scholar
- Gonzalez-Pajuelo M, Meynial-Salles I, Mendes F, Soucaille P, Vasconcelos I (2006) Microbial conversion of glycerol to 1,3-propanediol: physiological comparison of a natural producer, Clostridium butyricum VPI 3266, and an engineered strain, Clostridium acetobutylicum DG1(pSPD5). Appl Environ Microbiol 72(1):96–101. https://doi.org/10.1128/AEM.72.1.96-101.2006 CrossRefPubMedPubMedCentralGoogle Scholar
- Kanjilal B, Noshadi I, Bautista EJ, Srivastava R, Parnas RS (2015) Batch, design optimization, and DNA sequencing study for continuous 1,3-propanediol production from waste glycerol by a soil-based inoculum. Appl Microbiol Biotechnol 99(5):2105–2117. https://doi.org/10.1007/s00253-014-6259-5 CrossRefPubMedGoogle Scholar
- Loureiro-Pinto M, Coca M, González-Benito G, Lucas S, García-Cubero MT (2017) Continuous bioproduction of 1,3-propanediol from biodiesel raw glycerol: operation with free and immobilized cells of Clostridium butyricum DSM 10702. Can J Chem Eng 95(5):819–826. https://doi.org/10.1002/cjce.22725 CrossRefGoogle Scholar
- Menzel K, Zeng AP, Biebl H, Deckwer WD (1996) Kinetic, dynamic, and pathway studies of glycerol metabolism by Klebsiella pneumoniae in anaerobic continuous culture: I. The phenomena and characterization of oscillation and hysteresis. Biotechnol Bioeng 52(5):549–560. https://doi.org/10.1002/(SICI)1097-0290(19961205)52:5<549::AID-BIT2>3.0.CO;2-J CrossRefPubMedGoogle Scholar
- Mu XJ, Teng H, Sun YQ, Xiu ZL (2009) Techno-economic analysis on bioconversion of glycerol into 1,3-propanediol. Chin J Process Eng 9(5):947–952Google Scholar
- Papanikolaou S, Rontou M, Belka A, Athenaki M, Gardeli C, Mallouchos A, Kalantzi O, Koutinas A, Kookos I, Zeng AP, Aggelis G (2017) Conversion of biodiesel-derived glycerol into biotechnological products of industrial significance by yeast and fungal strains. Eng Life Sci 17(3):262–281. https://doi.org/10.1002/elsc.201500191 CrossRefGoogle Scholar
- Reimann A, Abbad-Andaloussi S, Biebl H, Petitdemange H (1998) 1,3-Propanediol formation with product-tolerant mutants of Clostridium butyricum DSM 5431 in continuous culture: productivity, carbon and electron flow. J Appl Microbiol 84(6):1125–1130. https://doi.org/10.1046/j.1365-2672.1998.00453.x CrossRefGoogle Scholar
- Ringel AK, Wilkens E, Hortig D, Willke T, Vorlop KD (2012) An improved screening method for microorganisms able to convert crudeglycerol to 1,3-propanediol and to tolerate high product concentrations. Appl Microbiol Biotechnol 93:1049–1056. https://doi.org/10.1007/s00253-011-3594-7 CrossRefPubMedGoogle Scholar
- Sittijunda S, Reungsang A (2017) Fermentation of hydrogen, 1,3-propanediol and ethanol from glycerol as affected by organic loading rate using up-flow anaerobic sludge blanket (UASB) reactor. Int J Hydrog Energy 42(45):27558–27569. https://doi.org/10.1016/j.ijhydene.2017.05.149 CrossRefGoogle Scholar
- Varrone C, Floriotis G, Heggeset TMB, Le SB, Markussen S, Skiadas IV, Gavala HN (2017) Continuous fermentation and kinetic experiments for the conversion of crude glycerol derived from second-generation biodiesel into 1,3-propanediol and butyric acid. Biochem Eng J 128:149–161. https://doi.org/10.1016/j.bej.2017.09.012 CrossRefGoogle Scholar
- Xiu ZL, Zeng AP (1999) A study progress in oscillatory and chaotic behavior in microbial continuous cultures. Prog Biotechnol 19(6):58–63Google Scholar
- Zeng AP, Menzel K, Deckwer W-D (1996) Kinetic, dynamic, and pathway studies of glycerol metabolism by Klebsiella pneumoniae in anaerobic continuous culture: II. Analysis of metabolic rates and pathways under oscillation and steady-state conditions. Biotechnol Bioeng 52(5):561–571. https://doi.org/10.1002/(SICI)1097-0290(19961205)52:5<561::AID-BIT3>3.0.CO;2-H CrossRefPubMedGoogle Scholar
- Zhou JJ, Shen JT, Jiang LL, Sun YQ, Mu Y, Xiu ZL (2017) Selection and characterization of an anaerobic microbial consortium with high adaptation to crude glycerol for 1,3-propanediol production. Appl Microbiol Biotechnol 101(15):5985–5996. https://doi.org/10.1007/s00253-017-8311-8 CrossRefPubMedGoogle Scholar