Two-stage partial nitritation-anammox process for high-rate mainstream deammonification
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Increasing information supported that achieving high-rate mainstream deammonification through two-stage partial nitritation (PN)-anammox process should be a better option than through single-stage process. However, direct experimental evidence was limited so far. Herein, a two-stage PN-anammox process was successfully operated for nitrogen removal from low-strength wastewater in winter. Influent shift from synthetic wastewater to actual anaerobically pretreated sewage had little impact on the process performance. Promising nitrogen removal rates (NRRs) of 0.28–0.07 kg N m−3 d−1 with an average effluent concentration of 5.2 mg TN L−1 were achieved for the anaerobically pretreated sewage treatment at 15–7 °C. Moreover, nearly all the degradable COD in the pretreated sewage was steadily removed in the first-stage PN reactor, despite the varied influent COD concentrations of 22–78 mg L−1 and the operating temperature decrease, suggesting the positive role of the first-stage PN in protecting anammox bacteria. The low temperature seemingly was the only deterministic factor inhibiting the anammox activity, and hence made the anammox reaction to be the rate-limiting step for nitrogen removal in the two-stage PN-anammox process. Unexpectedly, nearly all the anammox bacteria remained active at low temperatures with the process actual anammox activity reached about 76–85% of their maximum potential, implying that higher NRRs would be easily realized through bioaugmentation or enrichment of anammox bacteria. Overall, the present investigation provides direct and valuable information for implementing the two-stage PN-anammox process to treat mainstream municipal wastewater. A control strategy was also proposed to optimize the operation of the two-stage mainstream deammonification process.
KeywordsTwo-stage Partial nitritation Anammox Low temperature Sewage
This research was supported by Major Science and Technology Program for Water Pollution Control and Treatment (Grant No. 2013ZX07315–001) and National Natural Science Foundation of China (No. 51578353 and No. 51508366). Authors also acknowledge support from the Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- APHA (2005) Standard methods for the examination for water and wastewater American Public Health Association, 21th edn. American Public Health Association, Washington, DCGoogle Scholar
- De Clippeleir H, Vlaeminck SE, De Wilde F, Daeninck K, Mosquera M, Boeckx P, Verstraete W, Boon N (2013) One-stage partial nitritation/anammox at 15 C on pretreated sewage: feasibility demonstration at lab-scale. Appl Microb Biotechnol 97(23):10199–10210. https://doi.org/10.1007/s00253-013-4744-x CrossRefGoogle Scholar
- Guo Q, Xing BS, Li P, Xu JL, Yang CC, Jin RC (2015) Anaerobic ammonium oxidation (anammox) under realistic seasonal temperature variations: characteristics of biogranules and process performance. Bioresour Technol 192:765–773. https://doi.org/10.1016/j.biortech.2015.06.049 CrossRefPubMedGoogle Scholar
- Kouba V, Vejmelkova D, Proksova E, Wiesinger H, Concha M, Dolejs P, Hejnic J, Jenicek P, Bartacek J (2017) High-rate partial Nitritation of municipal wastewater after psychrophilic anaerobic pretreatment. Environ Sci Technol 51(19):11029–11038. https://doi.org/10.1021/acs.est.7b02078 CrossRefPubMedGoogle Scholar
- Laureni M, Falas P, Robin O, Wick A, Weissbrodt DG, Nielsen JL, Ternes TA, Morgenroth E, Joss A (2016) Mainstream partial nitritation and anammox: long-term process stability and effluent quality at low temperatures. Water Res 101:628–639. https://doi.org/10.1016/j.watres.2016.05.005 CrossRefPubMedPubMedCentralGoogle Scholar
- Leal CD, Pereira AD, Nunes FT, Ferreira LO, Coelho ACC, Bicalho SK, Mac Conell EFA, Ribeiro TB, de Lemos Chernicharo CA, de Araújo JC (2016) Anammox for nitrogen removal from anaerobically pre-treated municipal wastewater: effect of COD/N ratios on process performance and bacterial community structure. Bioresour Technol 211:257–266. https://doi.org/10.1016/j.biortech.2016.03.107 CrossRefPubMedGoogle Scholar
- Liu WR, Ji XM, Wang JF, Yang DH, Shen YL, Chen CJ, Qian FY, Wu P (2018) Microbial community response to influent shift and lowering temperature in a two-stage mainstream deammonification process. Bioresour Technol 262:132–140. https://doi.org/10.1016/j.biortech.2018.04.082 CrossRefPubMedGoogle Scholar
- Liu WR, Yang DH, Chen WJ, Gu X (2017) High-throughput sequencing-based microbial characterization of size fractionated biomass in an anoxic anammox reactor for low-strength wastewater at low temperatures. Bioresour Technol 231:45–52. https://doi.org/10.1016/j.biortech.2017.01.050 CrossRefPubMedGoogle Scholar
- Lotti T, Kleerebezem R, Hu Z, Kartal B, de Kreuk MK, Kip CET, Kruit J, Hendrickx TLG, van Loosdrecht MCM (2015a) Pilot-scale evaluation of anammox-based mainstream nitrogen removal from municipal wastewater. Environ Technol 36(9):1167–1177. https://doi.org/10.1080/09593330.2014.982722 CrossRefPubMedGoogle Scholar
- Malovanyy A, Yang J, Trela J, Plaza E (2015) Combination of upflow anaerobic sludge blanket (UASB) reactor and partial nitritation/anammox moving bed biofilm reactor (MBBR) for municipal wastewater treatment. Bioresour Technol 180:144–153. https://doi.org/10.1016/j.biortech.2014.12.101 CrossRefPubMedGoogle Scholar
- Wells GF, Shi Y, Laureni M, Rosenthal A, Szivak I, Weissbrodt DG, Joss A, Buergmann H, Johnson DR, Morgenroth E (2017) Comparing the resistance, resilience, and stability of replicate moving bed biofilm and suspended growth combined Nitritation-Anammox reactors. Environ Sci Technol 51(9):5108–5117. https://doi.org/10.1021/acs.est.6b05878 CrossRefPubMedGoogle Scholar