Applied Microbiology and Biotechnology

, Volume 102, Issue 15, pp 6687–6697 | Cite as

A novel technique for aflatoxin M1 detoxification using chitin or treated shrimp shells: in vitro effect of physical and kinetic parameters on the binding stability

  • Jean Claude AssafEmail author
  • André El Khoury
  • Ali Atoui
  • Nicolas Louka
  • Ali Chokr
Methods and protocols


This study aimed to investigate the ability of chitin and heat-treated shrimp shells to bind aflatoxin M1 (AFM1) in liquid matrix. Several concentrations of chitin or shrimp shells (grinded and ungrinded) were incubated in AFM1-contaminated phosphate-buffered saline (PBS) at different incubation times. The stability of the formed adsorbent-AFM1 complex was also tested in milk at different incubation times and temperatures. The unbound AFM1 was quantified by HPLC. Thereby, the percentages of the initial bounded AFM1 varied between 14.29 and 94.74%. Interestingly, in milk, an increase in incubation time coupled with a decrease in temperature affected positively the amount of bounded AFM1 to chitin and negatively those bounded to ungrinded shells. Results also revealed a partial reversibility in the binding of AFM1 to these adsorbents. These findings provided strong evidence on ability of chitin or shrimp shells by-product to bind AFM1 in milk and in PBS.


Aflatoxin M1 Chitin N-acetylglucosamine Shrimp shells Milk Binding 



This work was supported by the research and analysis center (CAR) and the research council at Saint-Joseph University (USJ).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with animals performed by any of the authors.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jean Claude Assaf
    • 1
    • 2
    • 3
    • 4
    Email author
  • André El Khoury
    • 1
  • Ali Atoui
    • 2
  • Nicolas Louka
    • 1
  • Ali Chokr
    • 2
    • 3
  1. 1.Centre d’Analyses et de Recherche (CAR), Unité de Recherche Technologies et Valorisation agro-Alimentaire (UR-TVA), Faculté des SciencesUniversité Saint-Joseph de BeyrouthBeirutLebanon
  2. 2.Laboratory of Microbiology, Department of Life and Earth Sciences, Faculty of Sciences ILebanese UniversityBeirutLebanon
  3. 3.Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and TechnologiesLebanese UniversityBeirutLebanon
  4. 4.Ecole Doctorale “Sciences et Santé”Université Saint-Joseph de BeyrouthBeirutLebanon

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