Monitoring the impact of an aspartic protease (MpAPr1) on grape proteins and wine properties
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The perception of haze in wine is brought about when pathogenesis-related proteins become unstable and aggregate, subsequently resulting in crosslinking until it develops into light-dispersing particles. Elimination of these proteins is usually achieved via bentonite fining, which, although effective, suffers from several drawbacks. The utilization of proteases has been proposed as an ideal alternative. In a previous study, an aspartic protease (MpAPr1) from the yeast Metschnikowia pulcherrima was purified and shown to be partially active against grape proteins in synthetic medium. In this study, the effects of pure MpAPr1 supplemented to Sauvignon Blanc juice on subsequent fermentation were investigated. The juice was incubated for 48 h and thereafter inoculated with Saccharomyces cerevisiae. Results revealed that the enzyme had no observable effects on fermentation performance and retained activity throughout. Protein degradation could be detected and resulted in a significant modification of the wine composition and an increase in the presence of certain volatile compounds, especially those linked to amino acid metabolism.
KeywordsMetschnikowia pulcherrima Aspartic protease Grape proteins Protein haze Wine
The authors thank the Central Analytical Facility, Stellenbosch University, for their technical support.
The author would like to thank the National Research Foundation of South Africa (UID: 88819) and Winetech for their financial support.
Compliance with ethical standards
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
The authors declare that they have no conflict of interest.
- Bakalinsky AT, Boulton R (1985) The study of an immobilized acid protease for the treatment of wine proteins. Am J Enol Vitic 36:23–29Google Scholar
- Bauer FF, Ndlovu T (2015) Method for preventing wine haze formation. South Africa Patent,Google Scholar
- Cabello-Pasini A, Victoria-Cota N, Carranza V, Garibay E, Salazar R (2005) Clarification of wines using polysaccharide extracted from seaweeds. Am J Enol Vitic 56:52–59Google Scholar
- Dizy M, Bisson LF (2000) Proteolytic activity of yeast strains during grape juice fermentation. Am J Enol Vitic 51:155–167Google Scholar
- Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD, Bairoch A (2005) Protein identification and analysis tools on the ExPASy server. In: Protein identification and analysis tools on ExPASy server. Humana PressGoogle Scholar
- Hsu JC, Heatherbell DA, Flores JH, Watson BT (1987) Heat unstable proteins in grape juice and wine. Am J Enol Vitic 38:17–22Google Scholar
- Lagace LS, Bisson LF (1990) Survey of yeast acid proteases for effectiveness of wine haze reduction. Am J Enol Vitic 41:147–155Google Scholar
- Macias S, Mateo JJ (2015) Enzyme contribution of non-Saccharomyces yeasts to wine production. J Microbiol Res 3:17–25Google Scholar
- Manteau S, Lambert B, Jeandet P, Legendre L (2003) Changes in chitinase and thaumatin-like pathogenesis-related proteins of grape berries during the champagne winemaking process. Am J Enol Vitic 54(4):267–272Google Scholar
- Marangon M, Sauvage FX, Waters EJ, Vernhet A (2011b) Effect of ionic strength and sulfate upon thermal aggregation of grape chitinases and thaumatin-like proteins in a model system. J Agric Food Chem 59:1157–1165Google Scholar
- Moio L, Ugliano M, Gambuti A, Genovese A, Piombino P (2004) Influence of clarification treatment on concentration of selected free varietal aroma compounds and glycoconjugates in Falanghina (Vitis vinifera L.) must and wine. Am J Enol Vitic 55:7–12Google Scholar
- Rollero S, Mouret JR, Bloem A, Sanchez I, Ortiz-Julien A, Sablayrolles JM, Dequin S, Camarasa C (2017) Quantitative 13C-isotope labelling-based analysis to elucidate the influence of environmental parameters on the production of fermentative aromas during wine fermentation. Microbial Biotechnol 10:1649–1662CrossRefGoogle Scholar
- Sanborn M, Edwards CG, Ross CF (2010) Impact of fining on chemical and sensory properties of Washington State Chardonnay and Gewurztraminer wine. Am J Enol Vitic 61:31–41Google Scholar
- Tal M, Silberstein A, Nusser E (1984) Why does Coomassie brilliant blue R interact differently with different proteins. J Biol Chem 260(18):9976–9980Google Scholar
- Theron LW, Divol B (2014) Microbial aspartic proteases: current and potential applications in industry. Appl Microbiol Biotechnol 98(21):8853–8868. https://doi.org/10.1007/s00253-014-6035-6
- Vincenzi S, Polesani M, Curioni A (2005) Removal of specific protein components by chitin enhances protein stability in a white wine. Am J Enol Vitic 56:246–254Google Scholar