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Applied Microbiology and Biotechnology

, Volume 102, Issue 11, pp 4937–4949 | Cite as

Extracellular polymer substance synthesized by a halophilic bacterium Chromohalobacter canadensis 28

  • Nadja Radchenkova
  • Ivanka Boyadzhieva
  • Nikolina Atanasova
  • Annarita Poli
  • Ilaria Finore
  • Paola Di Donato
  • Barbara Nicolaus
  • Ivan Panchev
  • Margarita Kuncheva
  • Margarita Kambourova
Environmental biotechnology

Abstract

Halophilic microorganisms are producers of a lot of new compounds whose properties suggest promising perspectives for their biotechnological exploration. Moderate halophilic bacterium Chromohalobacter canadensis 28 was isolated from Pomorie salterns as an extracellular polymer substance (EP) producer. The best carbon source for extracellular polymer production was found to be lactose, a sugar received as a by-product from the dairy industry. After optimization of the culture medium and physicochemical conditions for cultivation, polymer biosynthesis increased more than 2-fold. The highest level of extracellular polymer synthesis by C. canadensis 28 was observed in an unusually high NaCl concentration (15% w/v). Chemical analysis of the purified polymer revealed the presence of an exopolysaccharide (EPS) fraction (14.3% w/w) and protein fraction (72% w/w). HPLC analysis of the protein fraction showed the main presence of polyglutamic acid (PGA) (75.7% w/w). EPS fraction analysis revealed the following sugar composition (% w/w): glucosamine 36.7, glucose 32.3, rhamnose 25.4, xylose 1.7, and not identified sugar 3.9. The hydrogel formed by PGA and EPS fractions showed high swelling behavior, very good emulsifying and stabilizing properties, and good foaming ability. This is the first report for halophilic bacterium able to synthesize a polymer containing PGA fraction. The synthesized biopolymer shows an extremely high hydrophilicity, due to the simultaneous presence of PGA and EPS. The analysis of its functional properties and the presence of glucosamine in the highest proportion in EPS fraction clearly determine the potential of EP synthesized by C. canadensis 28 for application in the cosmetics industry.

Keywords

Halophilic bacteria Chromohalobacter canadensis Exopolymer synthesis Polyglutamic acid EPS 

Notes

Funding

This study was supported by the National Fund for Scientific Research, Bulgaria (Grant No. B02/26), and Joint Project session 2016–2018 of Bilateral Agreement between the Bulgarian Academy of Sciences (BAS), Bulgaria, and Consiglio Nazionale delle Ricerche (CNR), Italy.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2018_8901_MOESM1_ESM.pdf (321 kb)
ESM 1 (PDF 321 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Nadja Radchenkova
    • 1
  • Ivanka Boyadzhieva
    • 1
  • Nikolina Atanasova
    • 1
  • Annarita Poli
    • 2
  • Ilaria Finore
    • 2
  • Paola Di Donato
    • 2
  • Barbara Nicolaus
    • 2
  • Ivan Panchev
    • 3
  • Margarita Kuncheva
    • 3
  • Margarita Kambourova
    • 1
  1. 1.Institute of MicrobiologyBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Institute of Biomolecular ChemistryConsiglio Nazionale delle RicerchePozzuoliItaly
  3. 3.University of Food TechnologiesPlovdivBulgaria

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