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Spermidine plays a significant role in stabilizing a master transcription factor Clp to promote antifungal activity in Lysobacter enzymogenes

  • Yun Zhao
  • Tingting Zhang
  • Yang Ning
  • Danyu Shen
  • Nianda Yang
  • Yingying Li
  • Shan-Ho Chou
  • Liang Yang
  • Guoliang QianEmail author
Applied genetics and molecular biotechnology
  • 49 Downloads

Abstract

Spermidine is a common polyamine compound produced in bacteria, but its roles remain poorly understood. The bacterial SpeD encodes an S-adenosylmethionine decarboxylase that participates in spermidine synthesis. Lysobacter enzymogenes is an efficient environmental predator of crop fungal pathogens by secreting an antifungal antibiotic HSAF (heat-stable antifungal factor), while Clp is a master transcription factor essential for the antifungal activity of L. enzymogenes. In this work, we observed that speD was a close genomic neighbor of the clp gene. This genomic arrangement also seems to occur in many other bacteria, but the underlying reason remains unclear. By using L. enzymogenes OH11 as a working model, we showed that SpeD was involved in spermidine production that was essential for the L. enzymogenes antifungal activity. Spermidine altered the bacterial growth capability and HSAF production, both of which critically contributed to the L. enzymogenes antifungal activity. We further found that spermidine in L. enzymogenes was able to play a crucial, yet indirect role in maintaining the Clp level in vivo, at least partially accounting for its role in the antifungal activity. Thus, our findings suggested that spermidine probably plays an uncharacterized role in maintaining the levels of the master transcription regulator Clp to optimize its role in antifungal activity in an agriculturally beneficial bacterium.

Keywords

Lysobacter Polyamine Spermidine Antifungal activity Clp 

Notes

Acknowledgements

We thank Prof. Wei Qian from the Chinese Academy of Science for providing facilities in MST assay and the anti-Clp antibody.

Author contribution

S.C., L.Y., and G.Q. conceived the project and designed experiments. Y.Z., K.X., T.Z., D.S., S.H., Y.H., and L.Y. carried out experiments. Z.Y., S.D., L.Y., S.C, and G.Q. analyzed data. Z.Y., S.C., L.Y., and G.Q. wrote and revised the manuscript draft.

Funding information

This study was supported by the National Key Research and Development Program (2017YFD0201100 to G. Qian), the Fundamental Research Funds for the Central Universities (KYT201805, Y0201600126, and KYTZ201403 to GQ), the Jiangsu Agricultural Science and Technology Innovation Fund (CX(18)1003), Natural Science Fund of Jiangsu Province (BK20181325), the Innovation Team Program for Jiangsu Universities (2017), the National Ministry of Science and Technology of Taiwan (105-2113-M-005-013-MY2 to S. Chou), and the AcRF Tier 2 (MOE2016-T2-1-010 to L. Yang) from the Ministry of Education, Singapore. The funders have no role in study design.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This article does not contain any studies with human participants or animals.

Supplementary material

253_2018_9596_MOESM1_ESM.pdf (427 kb)
ESM 1 (PDF 426 kb)
253_2018_9596_MOESM2_ESM.xlsx (148 kb)
ESM 2 (XLSX 148 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests)Nanjing Agricultural UniversityNanjing CityPeople’s Republic of China
  2. 2.Singapore Centre for Environmental Life Sciences EngineeringNanyang Technological UniversitySingaporeSingapore
  3. 3.Institute of Biochemistry, and NCHU Agricultural Biotechnology CenterNational Chung Hsing UniversityTaichungTaiwan, Republic of China

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